Zentrum für Umweltforschung und nachhaltige Technologien, Fachbereich Biologie/Chemie, Abteilung Marine Mikrobiologie, Universität Bremen, D-28359 Bremen, Germany.
Syst Appl Microbiol. 2010 Apr;33(3):116-21. doi: 10.1016/j.syapm.2009.12.003. Epub 2010 Feb 12.
Four species of marine purple sulfur bacteria of the genus Marichromatium have been validly described. A recent re-analysis of the 16S rRNA-based similarity and genomic DNA-DNA hybridizations (DDH) of the type strains [33] suggested that some of them are so closely related that they can be considered heterotypic synonyms. Here, we report on the evaluation of the multilocus sequence analysis approach (MLSA) for nine Marichromatium strains in order to resolve their intrageneric genealogical relationships. MLSA was based on six protein-coding genes (gyrB, recA, fusA, dnaK, pufM, and soxB), and the results were comparable to DDH. The phylogenetic tree constructed with the concatenated sequences, which also included the 16S rRNA gene and the internal transcriber spacer ITS region (4331bp), separated the nine strains in four lineages that reflected the four Marichromatium species. The reconstructed phylogenetic tree based on concatenation of six protein-coding genes was also highly congruent with the tree topology based on the 16S rRNA gene.
已有效描述了四种海洋紫色硫细菌属马里chromatium。最近对[33]型菌株的基于 16S rRNA 的相似性和基因组 DNA-DNA 杂交（DDH）的重新分析表明，其中一些菌株非常密切相关，可以被视为异型同义词。在这里，我们报告了对九株马里chromatium 菌株进行多位点序列分析（MLSA）的评估，以解决其属内的系统发育关系。MLSA 基于六个编码蛋白的基因（gyrB、recA、fusA、dnaK、pufM 和 soxB），结果与 DDH 相媲美。用包含 16S rRNA 基因和内部转录间隔区 ITS 区（4331bp）的连接序列构建的系统发育树将这九株菌株分为四个谱系，反映了四种马里chromatium 物种。基于六个编码蛋白基因的连接构建的重建系统发育树也与基于 16S rRNA 基因的树拓扑高度一致。
