Department of Molecular and Cell Biology, University of Cape Town, Private Bag X3, Rondebosch, 7701 Cape Town, South Africa.
Syst Appl Microbiol. 2012 Oct;35(7):441-6. doi: 10.1016/j.syapm.2012.08.001. Epub 2012 Sep 13.
Multilocus sequence analysis (MLSA) was used to refine the phylogenetic analysis of the genus Kribbella, which currently contains 17 species with validly-published names. Sequences were obtained for the 16S rRNA, gyrB, rpoB, recA, relA and atpD genes for 16 of the 17 type strains of the genus plus seven non-type strains. A five-gene concatenated sequence of 4099 nt was used to examine the phylogenetic relationships between the species of the genus Kribbella. Using the concatenated sequence of the gyrB-rpoB-recA-relA and atpD genes, most Kribbella type strains can be distinguished by a genetic distance of >0.04. Each single-gene tree had an overall topology similar to that of the concatenated sequence tree. The single-gene relA tree, used here for the first time in MLSA of actinobacteria, had good bootstrap support, comparable to the rpoB and atpD gene trees, which had topologies closest to that of the concatenated sequence tree. This illustrates that relA is a useful addition in MLSA studies of the genus Kribbella. We propose that concatenated gyrB-rpoB-recA-relA-atpD gene sequences be used for examining the phylogenetic relationships within the genus Kribbella and for determining the closest phylogenetic relatives to be used for taxonomic comparisons.
多位点序列分析（MLSA）被用于完善 Kribbella 属的系统发育分析，该属目前包含 17 个具有有效发表名称的种。对该属的 17 个模式株中的 16 个以及 7 个非模式株的 16S rRNA、gyrB、rpoB、recA、relA 和 atpD 基因进行了测序。使用 4099nt 的 5 个基因串联序列来研究 Kribbella 属种间的系统发育关系。使用 gyrB-rpoB-recA-relA 和 atpD 基因的串联序列，大多数 Kribbella 模式株可以通过遗传距离>0.04 来区分。每个单基因树的总体拓扑结构与串联序列树的拓扑结构相似。在这里首次用于放线菌 MLSA 的单基因 relA 树具有良好的自举支持，与 rpoB 和 atpD 基因树相当，其拓扑结构与串联序列树最接近。这表明 relA 是 Kribbella 属 MLSA 研究中的一个有用补充。我们建议使用 gyrB-rpoB-recA-relA-atpD 基因串联序列来研究 Kribbella 属内的系统发育关系，并确定最接近的系统发育亲缘种用于分类比较。
