Department of Biology, Yonsei University, 262 Seongsanno, Seodaemun-gu, Seoul 120-749, Republic of Korea.
Biochem Biophys Res Commun. 2010 Mar 19;393(4):656-61. doi: 10.1016/j.bbrc.2010.02.047. Epub 2010 Feb 12.
We investigated cell shape changes during wound closure in the Drosophila larval epidermis. During reepithelialization, epidermal cells permanently change shape from pentagonal or hexagonal to irregular forms. This process requires zipper, a gene encoding the Drosophila nonmuscle myosin II heavy chain. Following wounding, myosin II is localized at the wound margin and at the rear end of individual cells located within several rows from the wound hole. The c-Jun N-terminal kinase (JNK) pathway is essential for this myosin II localization. These results suggest that not only the wound leading edge but also the cells lying distal to the leading edge cells actively participate in epithelial cell sheet migration during wound hole closure.
我们研究了果蝇幼虫表皮在伤口闭合过程中的细胞形状变化。在再上皮化过程中，表皮细胞从五边形或六边形永久地变为不规则形状。这个过程需要 zipper，它编码果蝇非肌肉肌球蛋白 II 重链。受伤后，肌球蛋白 II 定位于伤口边缘和位于离伤口孔几个细胞行内的单个细胞的后端。c-Jun N 端激酶（JNK）途径对于这种肌球蛋白 II 定位是必需的。这些结果表明，不仅伤口前缘，而且位于前缘细胞远处的细胞在伤口孔闭合期间积极参与上皮细胞片迁移。
