Department of Biology, Yonsei University, 262 Seongsanno, Seodaemun-gu, Seoul 120-749, Republic of Korea.
Biochem Biophys Res Commun. 2010 Apr 9;394(3):488-92. doi: 10.1016/j.bbrc.2010.02.124. Epub 2010 Feb 23.
A wound induces cell polarization, in which myosin II is localized at the rear end of individual cells in a migrating epithelial sheet of the Drosophila larval epidermis. Here, we use myosin localization to demonstrate that Rac1, Cdc42, and Rho1 are each required for cell polarization and directional sensing of the wound. The three GTPases are also required for actin cable formation at the wound leading edge. Rac1, Cdc42, and Rho1 act upstream of c-Jun N-terminal kinase (JNK) to organize actin assembly. These results highlight the similarities between the molecular mechanism of Drosophila wound healing and those of Drosophila embryonic dorsal closure and the chemotactic response of Dictyostelium and leukocytes.
伤口诱导细胞极化，在果蝇幼虫表皮的迁移上皮片中，肌球蛋白 II 定位于单个细胞的后端。在这里，我们利用肌球蛋白的定位来证明 Rac1、Cdc42 和 Rho1 都需要细胞极化和对伤口的定向感应。这三种 GTPase 还需要在伤口前缘形成肌动蛋白电缆。Rac1、Cdc42 和 Rho1 作用于 c-Jun N 端激酶（JNK）上游，以组织肌动蛋白组装。这些结果突出了果蝇伤口愈合的分子机制与果蝇胚胎背侧闭合和 Dictyostelium 和白细胞趋化反应的相似性。
