Department of Neurology, University of Texas Medical Branch, Galveston, TX, USA.
Biochem Biophys Res Commun. 2010 Mar 19;393(4):649-55. doi: 10.1016/j.bbrc.2010.02.046. Epub 2010 Feb 12.
Gold nanoparticles (GNPs) offer a great promise in biomedicine. Currently, there is no data available regarding the accumulation of nanoparticles in vivo after repeated administration. The purpose of the present study was to evaluate the bioaccumulation and toxic effects of different doses (40, 200, and 400 microg/kg/day) of 12.5 nm GNPs upon intraperitoneal administration in mice every day for 8 days. The gold levels in blood did not increase with the dose administered, whereas in all the organs examined there was a proportional increase on gold, indicating efficient tissue uptake. Although brain was the organ containing the lowest quantity of injected GNPs, our data suggest that GNPs are able to cross the blood-brain barrier and accumulate in the neural tissue. Importantly, no evidence of toxicity was observed in any of the diverse studies performed, including survival, behavior, animal weight, organ morphology, blood biochemistry and tissue histology. The results indicate that tissue accumulation pattern of GNPs depend on the doses administered and the accumulation of the particles does not produce sub-acute physiological damage.
金纳米颗粒（GNPs）在生物医药领域具有广阔的应用前景。目前，关于重复给药后纳米颗粒在体内的积累情况尚无数据。本研究旨在评估不同剂量（40、200 和 400μg/kg/天）的 12.5nm GNPs 经腹腔给药，每天一次，连续 8 天后，对小鼠的生物累积和毒副作用。血液中的金含量并未随给药剂量的增加而增加，而在所有检查的器官中，金的含量呈比例增加，表明组织摄取效率较高。尽管大脑是含有注射 GNPs 最少的器官，但我们的数据表明，GNPs 能够穿过血脑屏障并在神经组织中积累。重要的是，在进行的包括生存、行为、动物体重、器官形态、血液生化学和组织组织学在内的各种研究中，均未观察到毒性证据。结果表明，GNPs 的组织积累模式取决于给药剂量，并且颗粒的积累不会产生亚急性生理损伤。
