Thurmond R L, Dodd S W, Brown M F
Department of Chemistry, University of Arizona, Tucson 85721.
Biophys J. 1991 Jan;59(1):108-13. doi: 10.1016/S0006-3495(91)82203-2.
The role of lipid diversity in biomembranes is one of the major unsolved problems in biochemistry. One parameter of possible importance is the mean cross-sectional area occupied per lipid molecule, which may be related to formation of nonbilayer structures and membrane protein function. We have used 2H NMR spectroscopy to compare the properties of 1,2-diperdeuteriopalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE-d62) and 1,2-diperdeuteriopalmitoyl-sn-glycero-3-phosphocholine (DPPC-d62) in the L alpha phase. We find that DPPE has greater segmental order than DPPC, and that this increase in order is related to the smaller area per acyl chain found for DPPE. Values of the mean cross-sectional chain area are calculated using a simple diamond lattice model for the acyl chain configurational statistics, together with dilatometry data. The results obtained for the mean area per molecule are comparable with those from low angle x-ray diffraction studies.
脂质多样性在生物膜中的作用是生物化学领域主要的未解问题之一。一个可能重要的参数是每个脂质分子占据的平均横截面积，这可能与非双层结构的形成及膜蛋白功能有关。我们利用2H核磁共振光谱法比较了Lα相中1,2 - 二全氘代棕榈酰 - sn - 甘油 - 3 - 磷酸乙醇胺（DPPE - d62）和1,2 - 二全氘代棕榈酰 - sn - 甘油 - 3 - 磷酸胆碱（DPPC - d62）的性质。我们发现DPPE的链段有序度比DPPC更高，且这种有序度的增加与DPPE中每个酰基链较小的面积有关。平均横截面链面积的值是使用用于酰基链构型统计的简单菱形晶格模型以及膨胀计数据计算得出的。每个分子平均面积的结果与低角度X射线衍射研究的结果相当。
