通过2H核磁共振光谱法测定的磷脂分子区域。磷脂酰乙醇胺和磷脂酰胆碱的比较。
Molecular areas of phospholipids as determined by 2H NMR spectroscopy. Comparison of phosphatidylethanolamines and phosphatidylcholines.
作者信息
Thurmond R L, Dodd S W, Brown M F
机构信息
Department of Chemistry, University of Arizona, Tucson 85721.
出版信息
Biophys J. 1991 Jan;59(1):108-13. doi: 10.1016/S0006-3495(91)82203-2.
Abstract
The role of lipid diversity in biomembranes is one of the major unsolved problems in biochemistry. One parameter of possible importance is the mean cross-sectional area occupied per lipid molecule, which may be related to formation of nonbilayer structures and membrane protein function. We have used 2H NMR spectroscopy to compare the properties of 1,2-diperdeuteriopalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE-d62) and 1,2-diperdeuteriopalmitoyl-sn-glycero-3-phosphocholine (DPPC-d62) in the L alpha phase. We find that DPPE has greater segmental order than DPPC, and that this increase in order is related to the smaller area per acyl chain found for DPPE. Values of the mean cross-sectional chain area are calculated using a simple diamond lattice model for the acyl chain configurational statistics, together with dilatometry data. The results obtained for the mean area per molecule are comparable with those from low angle x-ray diffraction studies.
摘要
脂质多样性在生物膜中的作用是生物化学领域主要的未解问题之一。一个可能重要的参数是每个脂质分子占据的平均横截面积，这可能与非双层结构的形成及膜蛋白功能有关。我们利用2H核磁共振光谱法比较了Lα相中1,2 - 二全氘代棕榈酰 - sn - 甘油 - 3 - 磷酸乙醇胺（DPPE - d62）和1,2 - 二全氘代棕榈酰 - sn - 甘油 - 3 - 磷酸胆碱（DPPC - d62）的性质。我们发现DPPE的链段有序度比DPPC更高，且这种有序度的增加与DPPE中每个酰基链较小的面积有关。平均横截面链面积的值是使用用于酰基链构型统计的简单菱形晶格模型以及膨胀计数据计算得出的。每个分子平均面积的结果与低角度X射线衍射研究的结果相当。
相似文献
1
Molecular areas of phospholipids as determined by 2H NMR spectroscopy. Comparison of phosphatidylethanolamines and phosphatidylcholines.通过2H核磁共振光谱法测定的磷脂分子区域。磷脂酰乙醇胺和磷脂酰胆碱的比较。
Biophys J. 1991 Jan;59(1):108-13. doi: 10.1016/S0006-3495(91)82203-2.
2
Phospholipid headgroups govern area per lipid and emergent elastic properties of bilayers.磷脂头部基团控制双层的脂质面积和弹性性质。
Biophys J. 2022 Nov 1;121(21):4205-4220. doi: 10.1016/j.bpj.2022.09.005. Epub 2022 Sep 9.
3
Area per lipid and acyl length distributions in fluid phosphatidylcholines determined by (2)H NMR spectroscopy.通过（2）H核磁共振光谱法测定的流体磷脂酰胆碱中每个脂质的面积和酰基长度分布。
Biophys J. 2000 Dec;79(6):3172-92. doi: 10.1016/S0006-3495(00)76551-9.
4
Curvature, order, and dynamics of lipid hexagonal phases studied by deuterium NMR spectroscopy.通过氘核磁共振光谱研究脂质六方相的曲率、有序性和动力学。
Biochemistry. 1993 May 25;32(20):5394-410. doi: 10.1021/bi00071a015.
5
Curcumin disorders 1,2-dipalmitoyl-sn-glycero-3-phosphocholine membranes and favors the formation of nonlamellar structures by 1,2-dielaidoyl-sn-glycero-3-phosphoethanolamine.姜黄素扰乱 1,2-二棕榈酰-sn-甘油-3-磷酸胆碱膜，并通过 1,2-二油酰基-sn-甘油-3-磷酸乙醇胺有利于形成非层状结构。
J Phys Chem B. 2010 Aug 5;114(30):9778-86. doi: 10.1021/jp101045p.
6
Acyl chain conformational ordering in 1,2 dipalmitoylphosphatidylethanolamine. Integration of FT-IR and 2H NMR results.1,2-二棕榈酰磷脂酰乙醇胺中酰基链的构象有序性。傅里叶变换红外光谱（FT-IR）和氘核磁共振（2H NMR）结果的整合。
Biophys J. 1992 Oct;63(4):1059-62. doi: 10.1016/S0006-3495(92)81676-4.
7
Effects of lipid chain unsaturation and headgroup type on molecular interactions between paclitaxel and phospholipid within model biomembrane.脂质链不饱和度和头部基团类型对模型生物膜中紫杉醇与磷脂分子间相互作用的影响。
J Colloid Interface Sci. 2005 May 1;285(1):326-35. doi: 10.1016/j.jcis.2004.11.032.
8
Correlation between lipid plane curvature and lipid chain order.脂质平面曲率与脂质链有序性之间的相关性。
Biophys J. 1996 Jun;70(6):2747-57. doi: 10.1016/S0006-3495(96)79844-2.
9
Acyl chain dynamics of phosphatidylethanolamines containing oleic acid and dihydrosterculic acid: 2H NMR relaxation studies.含油酸和二氢硬脂酸的磷脂酰乙醇胺的酰基链动力学：2H NMR弛豫研究
Biochemistry. 1985 Aug 13;24(17):4659-65. doi: 10.1021/bi00338a027.
10
Molecular simulation study of structural and dynamic properties of mixed DPPC/DPPE bilayers.二棕榈酰磷脂酰胆碱/二棕榈酰磷脂酰乙醇胺混合双层膜结构与动力学性质的分子模拟研究
Biophys J. 2006 Jun 1;90(11):3951-65. doi: 10.1529/biophysj.105.076596. Epub 2006 Mar 13.
引用本文的文献
1
Molecular Dynamics Simulation of Antimicrobial Peptide CM15 in and Model Bilayer Lipid.抗菌肽CM15在[具体内容缺失]和模型双层脂质中的分子动力学模拟
Iran J Biotechnol. 2023 Apr 1;21(2):e3344. doi: 10.30498/ijb.2023.337246.3344. eCollection 2023 Apr.
2
Molecular Dynamics Simulation of Transport Mechanism of Graphene Quantum Dots through Different Cell Membranes.石墨烯量子点通过不同细胞膜传输机制的分子动力学模拟
Membranes (Basel). 2022 Jul 31;12(8):753. doi: 10.3390/membranes12080753.
3
Molecular dynamics modeling of Pseudomonas aeruginosa outer membranes.铜绿假单胞菌外膜的分子动力学建模。
Phys Chem Chem Phys. 2018 Sep 19;20(36):23635-23648. doi: 10.1039/c8cp04278k.
4
Drude Polarizable Force Field for Molecular Dynamics Simulations of Saturated and Unsaturated Zwitterionic Lipids.用于饱和与不饱和两性离子脂质分子动力学模拟的德鲁德可极化力场
J Chem Theory Comput. 2017 Sep 12;13(9):4535-4552. doi: 10.1021/acs.jctc.7b00262. Epub 2017 Aug 8.
5
Cholesterol-induced suppression of membrane elastic fluctuations at the atomistic level.胆固醇在原子水平上对膜弹性波动的抑制作用。
Chem Phys Lipids. 2016 Sep;199:39-51. doi: 10.1016/j.chemphyslip.2016.05.001. Epub 2016 May 3.
6
Melittin-Induced Lipid Extraction Modulated by the Methylation Level of Phosphatidylcholine Headgroups.蜂毒肽诱导的脂质提取受磷脂酰胆碱头部基团甲基化水平的调节。
Biophys J. 2016 Jan 19;110(2):400-410. doi: 10.1016/j.bpj.2015.12.004.
7
Membrane interaction of antimicrobial peptides using E. coli lipid extract as model bacterial cell membranes and SFG spectroscopy.以大肠杆菌脂质提取物作为模型细菌细胞膜，利用表面荧光基团标记光谱法研究抗菌肽与膜的相互作用。
Chem Phys Lipids. 2015 Apr;187:20-33. doi: 10.1016/j.chemphyslip.2015.02.003. Epub 2015 Feb 20.
8
Ceramide-lipid interactions studied by MD simulations and solid-state NMR.通过分子动力学模拟和固态核磁共振研究神经酰胺与脂质的相互作用。
Biochim Biophys Acta. 2014 Oct;1838(10):2511-9. doi: 10.1016/j.bbamem.2014.05.024. Epub 2014 May 29.
9
The final frontier of pH and the undiscovered country beyond.pH 的终极边疆和未被发现的彼岸之国。
PLoS One. 2012;7(9):e45832. doi: 10.1371/journal.pone.0045832. Epub 2012 Sep 25.
10
Malleability of the folding mechanism of the outer membrane protein PagP: parallel pathways and the effect of membrane elasticity.外膜蛋白 PagP 折叠机制的可变性：平行途径和膜弹性的影响。
J Mol Biol. 2012 Feb 24;416(3):453-64. doi: 10.1016/j.jmb.2011.12.039. Epub 2012 Jan 8.
本文引用的文献
1
Dilatometry and calorimetry of saturated phosphatidylethanolamine dispersions.饱和磷脂酰乙醇胺分散体的膨胀测定法和量热法。
Biochemistry. 1981 Jan 6;20(1):187-92. doi: 10.1021/bi00504a031.
2
A statistical mechanical treatment of fatty acyl chain order in phospholipid bilayers and correlation with experimental data. A. Theory.磷脂双层中脂肪酰链有序性的统计力学处理及其与实验数据的相关性。A. 理论。
Biochim Biophys Acta. 1981 Jul 20;645(2):183-92. doi: 10.1016/0005-2736(81)90189-9.
3
Lipid conformation in model membranes and biological membranes.模型膜和生物膜中的脂质构象。
Q Rev Biophys. 1980 Feb;13(1):19-61. doi: 10.1017/s0033583500000305.
4
Molecular dynamics and conformation in the gel and liquid-crystalline phases of phosphatidylethanolamine bilayers.磷脂酰乙醇胺双层膜凝胶相和液晶相中的分子动力学与构象
Biochemistry. 1982 Nov 23;21(24):6220-30. doi: 10.1021/bi00267a030.
5
Interactions between neutral phospholipid bilayer membranes.中性磷脂双分子层膜之间的相互作用。
Biophys J. 1982 Mar;37(3):657-65.
6
Phase equilibria, molecular conformation, and dynamics in phosphatidylcholine/phosphatidylethanolamine bilayers.磷脂酰胆碱/磷脂酰乙醇胺双层膜中的相平衡、分子构象和动力学。
Biochemistry. 1982 Nov 23;21(24):6243-53. doi: 10.1021/bi00267a032.
7
A method for the synthesis of isomerically pure saturated mixed-chain phosphatidylcholines.一种合成异构体纯的饱和混合链磷脂酰胆碱的方法。
Anal Biochem. 1981 May 1;113(1):96-101. doi: 10.1016/0003-2697(81)90049-x.
8
Dynamic structure and phase behavior of dimyristoylphosphatidylethanolamine bilayers studied by deuterium nuclear magnetic resonance.通过氘核磁共振研究二肉豆蔻酰磷脂酰乙醇胺双层膜的动态结构和相行为。
Biochemistry. 1983 Jun 7;22(12):3023-6. doi: 10.1021/bi00281a036.
9
The description of membrane lipid conformation, order and dynamics by 2H-NMR.通过2H-NMR对膜脂构象、有序性和动力学的描述。
Biochim Biophys Acta. 1983 Mar 21;737(1):117-71. doi: 10.1016/0304-4157(83)90015-1.
10
Transphosphatidylation by phospholipase D.磷脂酶D介导的转磷脂酰基作用
J Biol Chem. 1967 Feb 10;242(3):477-84.
Zotero 插件