Vergara J, DiFranco M, Compagnon D, Suarez-Isla B A
Department of Physiology, University of California, Los Angeles 90024.
Biophys J. 1991 Jan;59(1):12-24. doi: 10.1016/S0006-3495(91)82193-2.
Epifluorescence images of Ca2+ transients elicited by electrical stimulation of single skeletal muscle fibers were studied with fast imaging techniques that take advantage of the large fluorescence signals emitted at relatively long wavelengths by the dyes fluo-3 and rhod-2 in response to binding of Ca2+ ions, and of the suitable features of a commercially available CCD video camera. The localized release of Ca2+ in response to microinjection of InsP3 was also monitored to demonstrate the adequate space and time resolutions of the imaging system. The time resolution of the imager system, although limited to the standard video frequency response, still proved to be adequate to investigate the fast Ca2+ release process in skeletal muscle fibers at low temperatures.
利用荧光素-3和罗丹明-2等染料在结合钙离子时在相对较长波长处发射的大荧光信号以及商用CCD摄像机的合适特性的快速成像技术，研究了电刺激单个骨骼肌纤维引发的钙离子瞬变的落射荧光图像。还监测了因注射肌醇三磷酸而引起的钙离子局部释放，以证明成像系统具有足够的空间和时间分辨率。成像系统的时间分辨率虽然限于标准视频频率响应，但仍被证明足以在低温下研究骨骼肌纤维中的快速钙离子释放过程。
