Lab. de Micología Experimental, Dpto. de Biodiversidad y Biología Experimental, Fac. Cs. Exactas y Naturales, PROPLAME - PRHIDEB - CONICET, Universidad de Buenos Aires, C1428EHA Ciudad Universitaria, CABA, Argentina.
Bioresour Technol. 2010 Jun;101(12):4554-63. doi: 10.1016/j.biortech.2010.01.102. Epub 2010 Feb 13.
The effect of amino acids, complex nitrogen sources and vitamin addition on Trametes trogii, Trametes villosa and Coriolus versicolor var. antarcticus ligninolytic enzyme production, was evaluated. Dye decolorization by their culture filtrates was compared. Glutamic acid followed by peptone, were the best N sources for laccase and manganese peroxidase production. The three fungi produced two laccase isoenzymes (molecular weights from 38 up to 150 kDa); their pattern of production was not affected by medium composition. Although the response was not uniform, vitamin addition sometimes stimulated ligninolytic enzyme production, but never inhibited it. Thiamine induced manganese peroxidase production. T. trogii grown in glutamic acid produced culture filtrates with the highest laccase (188.3 U/ml) and manganese peroxidase activities (4.5 U/ml), rendering the best results in decolorization. These crude filtrates were able to decolorize in half hour (at pH 4.5, 30 degrees C): 13%, 23%, 40%, 46%, 82%, 94% and 95% of Gentian Violet, Xylidine, Congo Red, Malachite Green, Remazol Brilliant Blue R, Indigo Carmine and Anthraquinone Blue, respectively.
评估了氨基酸、复合氮源和维生素添加对糙皮侧耳、栓菌和南极假丝酵母木质素降解酶产生的影响。比较了它们的培养液对染料的脱色效果。谷氨酸和蛋白胨是漆酶和锰过氧化物酶产生的最佳氮源。三种真菌产生了两种漆酶同工酶（分子量从 38 到 150 kDa 不等）；其产生模式不受培养基组成的影响。虽然反应不一致，但维生素添加有时会刺激木质素降解酶的产生，但从未抑制过它。硫胺素诱导锰过氧化物酶的产生。在谷氨酸中生长的 T. trogii 产生的培养液具有最高的漆酶（188.3 U/ml）和锰过氧化物酶活性（4.5 U/ml），在脱色方面效果最好。这些粗滤过液在半小时内能够将以下染料脱色：结晶紫 13%、二甲苯 23%、刚果红 40%、孔雀绿 46%、丽春红 BR 82%、靛蓝胭脂红 94%和蒽醌蓝 95%，条件为 pH 4.5、30°C。
