Environmental Microbiology and Biotechnology Research Center at Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2011 Jan;61(Pt 1):71-75. doi: 10.1099/ijs.0.020842-0. Epub 2010 Feb 12.
A bacterial strain, designated V2M29(T), was isolated from forest soil collected in the Changbai Mountains, Heilongjiang Province, China. Cells of strain V2M29(T) were Gram-positive, rod-shaped (0.5-0.8 μm in diameter and 1.5-2.0 μm in length), strictly aerobic and non-motile. Colonies produced on R2A agar plates were creamy, smooth, circular and 0.3-0.8 mm in diameter. Strain V2M29(T) grew at 14-35 °C (optimum, 29 °C), at pH 4.1-10.0 (optimum, pH 7.0-8.0) and in the presence of 0-7.0 % (w/v) NaCl (optimum, 0-2.0 %). Strain V2M29(T) contained MK-8(H(4)) as the major respiratory quinone and iso-C(15 : 0) (20.9 %), iso-C(16 : 0) (18.5 %), C(17 : 1)ω8c (16.7 %) and 10-methyl-C(17 : 0) (9.2 %) as the major cellular fatty acids. The cell-wall peptidoglycan type was based on meso-2,6-diaminopimelic acid and the acyl type of the muramic acid was acetyl. Diphosphatidylglycerol, phosphatidylinositol and six unknown glycolipids were the major polar lipids. The G+C content of the DNA was 72.0 mol% (T(m)). 16S rRNA gene sequence analysis indicated that strain V2M29(T) was phylogenetically related to members of the genus Phycicoccus, with sequence similarities ranging from 96.6-97.8 %. The DNA-DNA relatedness values of strain V2M29(T) to Phycicoccus dokdonensis DS-8(T) and Phycicoccus bigeumensis DSM 19264(T) were 32.5±3.3 % and 29.2±2.3 %, respectively. Based on these results, it is concluded that strain V2M29(T) represents a novel species of the genus Phycicoccus, for which the name Phycicoccus cremeus sp. nov. is proposed, with V2M29(T) (=CGMCC 1.6963(T) =NBRC 104261(T)) as the type strain.
一株细菌菌株，命名为 V2M29(T)，从中国黑龙江省长白山采集的森林土壤中分离得到。V2M29(T)菌株的细胞呈革兰氏阳性，杆状（直径 0.5-0.8μm，长度 1.5-2.0μm），严格需氧，不运动。在 R2A 琼脂平板上产生的菌落呈奶油色、光滑、圆形，直径为 0.3-0.8mm。V2M29(T)菌株在 14-35°C（最佳温度 29°C）、pH4.1-10.0（最佳 pH7.0-8.0）和 0-7.0%（w/v）NaCl 存在下生长（最佳 0-2.0%）。V2M29(T)菌株含有 MK-8(H(4))作为主要的呼吸醌和异-C(15 : 0)（20.9%）、异-C(16 : 0)（18.5%）、C(17 : 1)ω8c（16.7%）和 10-甲基-C(17 : 0)（9.2%）作为主要的细胞脂肪酸。细胞壁肽聚糖类型基于 meso-2,6-二氨基庚二酸，菌壁酸的酰基类型为乙酰基。双磷脂酰甘油、磷脂酰肌醇和六种未知糖脂是主要的极性脂。DNA 的 G+C 含量为 72.0mol%（T(m)）。16S rRNA 基因序列分析表明，V2M29(T)菌株与 Phycicoccus 属的成员具有系统发育关系，序列相似度在 96.6-97.8%之间。V2M29(T)菌株与 Phycicoccus dokdonensis DS-8(T)和 Phycicoccus bigeumensis DSM 19264(T)的 DNA-DNA 相关值分别为 32.5±3.3%和 29.2±2.3%。基于这些结果，可以得出结论，V2M29(T)菌株代表 Phycicoccus 属的一个新种，提议将其命名为 Phycicoccus cremeus sp. nov.，V2M29(T)（=CGMCC 1.6963(T)=NBRC 104261(T)）为其模式菌株。
