Jones K J, Pfaff D W, McEwen B S
Chicago Medical School, IL 60064.
Brain Res Bull. 1991 Jan;26(1):181-4. doi: 10.1016/0361-9230(91)90207-z.
Recent evidence suggests that E2 may act in the ventromedial nucleus of the hypothalamus (VMN) and arcuate nucleus (ARC) through mechanisms partly unique to each area. To examine this hypothesis, we investigated early and discontinuous effects of E2 action on neurons within the ARC from an ultrastructural and morphometric perspective. In contrast to our findings of dramatic, rapid effects of E2 on the structure of VMN neurons, no changes in any of the ultrastructural parameters examined, including the nucleolus, nucleus, nucleoplasm, and the nuclear envelope-RER system, were observed in ARC neurons after either 2 hours of E2 or a discontinuous schedule of 2 h on/7 h off/2 h on of E2. No changes were noted in any of the quantitative parameters examined, including nucleolar, nuclear, and somal area, nuclear shape, and nuclear envelope length after either 2 hours of E2 or a discontinuous schedule of 2 h on/7 h off/2 h on of E2. These morphological results support the hypothesis of differential actions of E2 on various E2-concentrating nuclear groups in the brain.
最近的证据表明，雌激素（E2）可能在下丘脑腹内侧核（VMN）和弓状核（ARC）中通过各区域部分独特的机制发挥作用。为了验证这一假设，我们从超微结构和形态计量学角度研究了E2作用于ARC内神经元的早期和间断性效应。与我们发现E2对VMN神经元结构有显著快速影响不同，在给予E2 2小时或采用2小时开启/7小时关闭/2小时开启的间断性E2给药方案后，ARC神经元中所检测的任何超微结构参数，包括核仁、细胞核、核质以及核膜 - 粗面内质网系统，均未观察到变化。在给予E2 2小时或采用2小时开启/7小时关闭/2小时开启的间断性E2给药方案后，所检测的任何定量参数，包括核仁、细胞核和体细胞区域、细胞核形状以及核膜长度，均未发现变化。这些形态学结果支持了E2对大脑中不同E2聚集核团具有差异性作用的假设。
