Faculty of Oral and Dental Medicine Cairo University, Dept. of Operative Dentistry, Manial, Cairo, Egypt.
J Adhes Dent. 2010 Feb;12(1):63-70. doi: 10.3290/j.jad.a17594.
To evaluate the effect of 6 irrigant regimens on the microshear bond strength of different adhesives to pulp chamber dentin and to determine their interfacial morphology by SEM.
Forty-three human molars were split into halves and ground to provide flat pulp chamber dentin. Eighty-five halves were used. The specimens were assigned to 6 groups according to the irrigant regimen used. Group I (n=10) was irrigated with distilled water (control). The remaining 5 groups (n=15 each) were irrigated with 5.25% NaOCl followed by either 5.25% NaOCl, 17% EDTA, benzalkonium chloride (Tubilcid Plus) or MTAD (Biopure), either rinsed or not. Irrigated specimens were bonded using Clearfil S3 Bond or Adper Single Bond 2, which was employed with or without etching. Resin composite cylinders were bonded using tygon tubes for microshear bond strength testing. Data were analyzed using two-way ANOVA followed by Tukey's post-hoc test. Representative specimens of each group were prepared from 17 further molars for interfacial SEM examination.
The different irrigant regimens used did not significantly alter the microshear bond strength values using either of the bonding systems, except NaOCl, which significantly reduced the microshear bond strength values with Adper Single Bond 2. Bonding irrigated specimens directly using the Adper Single Bond 2 without prior etching provided significantly reduced values.
The irrigant regimens examined could be used safely prior to bonding except for the NaOCl, which should be negated if it is to be followed by Adper Single Bond 2, and the etching step cannot be omitted if an etch-and-rinse adhesive system is the adhesive of choice.
评估 6 种冲洗方案对不同粘结剂与牙髓腔牙本质间微剪切粘结强度的影响，并通过 SEM 观察其界面形态。
43 颗人磨牙随机均分为两半，磨成平面状牙髓腔牙本质。共 85 个样本。根据冲洗方案的不同，将样本分为 6 组。第 1 组（n=10）用蒸馏水冲洗（对照组）。其余 5 组（n=15）分别用 5.25%次氯酸钠冲洗，之后分别用 5.25%次氯酸钠、17%乙二胺四乙酸、苯扎氯铵（Tubilcid Plus）或 MTAD（Biopure）冲洗，或不冲洗。冲洗后的样本分别用 Clearfil S3 粘结剂或 Adper Single Bond 2 粘结剂粘结，粘结时有的不进行酸蚀，有的进行酸蚀。用 Tygon 管粘结树脂复合圆柱体，进行微剪切粘结强度测试。采用双因素方差分析和 Tukey 事后检验对数据进行分析。从另外 17 颗磨牙中各取 1 个样本代表每组进行界面 SEM 观察。
除了次氯酸钠，其余冲洗方案对两种粘结系统的微剪切粘结强度值均无显著影响。单独使用 Adper Single Bond 2 时，次氯酸钠会显著降低粘结强度值。不进行酸蚀而直接使用 Adper Single Bond 2 粘结冲洗后的样本，其粘结强度值显著降低。
除了次氯酸钠（如果后续要用 Adper Single Bond 2，则应弃用），其余冲洗方案在粘结前均可安全使用。如果选择自酸蚀粘结剂系统，则不能省略酸蚀步骤。
