School of Pharmacy, Fudan University, Shanghai 200032, China.
Anal Bioanal Chem. 2010 Apr;396(7):2715-20. doi: 10.1007/s00216-010-3490-5. Epub 2010 Feb 14.
A simple method based on electric heating wires has been developed for the rapid fabrication of poly(methyl methacrylate) (PMMA) electrophoresis microchips in ordinary laboratories without the need for microfabrication facilities. A piece of stretched electric heating wire placed across the length of a PMMA plate along its midline was sandwiched between two microscope slides under pressure. Subsequently, alternating current was allowed to pass through the wire to generate heat to emboss a separation microchannel on the PMMA separation channel plate at room temperature. The injection channel was fabricated using the same procedure on a PMMA sheet that was perpendicular to the separation channel. The complete microchip was obtained by bonding the separation channel plate to the injection channel sheet, sealing the channels inside. The electric heating wires used in this work not only generated heat; they also served as templates for embossing the microchannels. The prepared microfluidic microchips have been successfully employed in the electrophoresis separation and detection of ions in connection with contactless conductivity detection.
已经开发出一种基于电热丝的简单方法，可在普通实验室中快速制造聚甲基丙烯酸甲酯（PMMA）电泳微芯片，而无需微制造设备。将一段拉伸的电热丝沿 PMMA 板的中线放置在其长度上，然后在压力下将其夹在两个显微镜载玻片之间。随后，允许交流电通过电线以在室温下在 PMMA 分离通道板上压印分离微通道。通过与分离通道垂直的 PMMA 片上的相同程序制造进样通道。通过将分离通道板粘合到进样通道片上，密封通道内部，即可获得完整的微芯片。本工作中使用的电热丝不仅产生热量，还可用作压印微通道的模板。所制备的微流控微芯片已成功用于与非接触式电导检测相结合的离子电泳分离和检测。
