建立化学发光酶联免疫吸附分析法用于橙汁、甘蓝和黄瓜中涕灭威和克百威的筛选。
Development of chemiluminescence enzyme-linked immunosorbent assay for the screening of metolcarb and carbaryl in orange juice, cabbage and cucumber.
机构信息
Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China.
出版信息
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2010 Mar;27(3):338-46. doi: 10.1080/19440040903403016.
A direct competitive enzyme-linked immunosorbent assay (ELISA) with enhanced chemiluminescent detection for simultaneous detection of metolcarb and carbaryl in agricultural products is reported. The concentrations of coating antibodies and enzyme tracer, as well as the effects of pH and methanol concentrations, were investigated. Under optimum conditions, the IC(50) of metolcarb and carbaryl were 4 and 0.3 microg kg(-1), respectively. Detection of metolcarb and carbaryl in fruit juices and vegetables was accomplished using simple, rapid and efficient extraction methods. Recoveries (n = 3) of spiked samples ranged 75-108% for metolcarb and 74-98% for carbaryl. Validation of the enhanced chemiluminescent immunosorbent assay was conducted by comparison of results with HPLC. The correlation between the data obtained using ELISA with enhanced chemiluminescent detection and HPLC was good (n = 9). Therefore, the enhanced chemiluminescent ELISA was suitable for quantitative simultaneously determination of metolcarb and carbaryl residue in agricultural products.
本文报道了一种用于同时检测农产品中甲萘威和西维因的竞争酶联免疫吸附测定法（ELISA），该方法具有增强化学发光检测功能。考察了包被抗体和酶标记物的浓度以及 pH 值和甲醇浓度的影响。在最佳条件下，甲萘威和西维因的 IC（50）分别为 4 和 0.3 μg kg（-1）。采用简单、快速、高效的提取方法，实现了果汁和蔬菜中甲萘威和西维因的检测。甲萘威和西维因的加标样品回收率（n = 3）分别为 75-108%和 74-98%。通过与 HPLC 的结果比较，对增强化学发光免疫吸附测定法的验证表明，ELISA 与增强化学发光检测法的数据相关性良好（n = 9）。因此，增强化学发光 ELISA 适用于定量同时检测农产品中甲萘威和西维因的残留。
Zotero 插件