采用酶联免疫吸附测定法测定农产品中的拟除虫菊酯残留量。

Determination of pyrethroid residues in agricultural products by an enzyme-linked immunosorbent assay.

作者信息

Park Eun-Kee, Kim Jung-Hyun, Gee Shirley J, Watanabe Takaho, Ahn Ki Chang, Hammock Bruce D

机构信息

Department of Entomology and UCD Cancer Research Center, University of California, Davis, California 95616, USA.

出版信息

J Agric Food Chem. 2004 Sep 8;52(18):5572-6. doi: 10.1021/jf049438z.

DOI:10.1021/jf049438z

PMID:15373394

Abstract

To determine cypermethrin and permethrin in agricultural products, a competitive enzyme-linked immunosorbent assay (ELISA) method was employed. The matrix interferences were minimized by direct dilution of the extracts. No further cleanup was needed. A minimum matrix effect with a 1:10 dilution of white wine for cypermethrin and a 1:200 dilution of red and white wines, fruits, and vegetables for permethrin was found when phosphate-buffered saline containing 40% methanol was employed as the diluent. Good recoveries of spiked levels were observed. The mean percentage recoveries of cypermethrin spiked in white wine and permethrin spiked in red and white wines were 99.7, 74, and 78%, respectively. The mean percentage recoveries of permethrin spiked in apple, banana, cucumber, lettuce, onion, and peach were 99.2, 105, 70.2, 97.5, 94.4, and 89.4%, respectively. Validation of the ELISA method with permethrin-spiked lettuce and peach was carried out using gas chromatography with mass spectrometry, resulting in a good recovery and correlation.

摘要

为测定农产品中的氯氰菊酯和氯菊酯，采用了竞争性酶联免疫吸附测定（ELISA）方法。通过直接稀释提取物将基质干扰降至最低。无需进一步净化。当使用含40%甲醇的磷酸盐缓冲盐水作为稀释剂时，发现氯氰菊酯用白葡萄酒1:10稀释、氯菊酯用红葡萄酒、白葡萄酒、水果和蔬菜1:200稀释时基质效应最小。加标水平的回收率良好。白葡萄酒中加标氯氰菊酯以及红葡萄酒和白葡萄酒中加标氯菊酯的平均回收率分别为99.7%、74%和78%。苹果、香蕉、黄瓜、生菜、洋葱和桃子中加标氯菊酯的平均回收率分别为99.2%、105%、70.2%、97.5%、94.4%和89.4%。使用气相色谱 - 质谱联用对加标氯菊酯的生菜和桃子进行ELISA方法验证，回收率和相关性良好。