Petru Poni Institute of Macromolecular Chemistry, Aleea Grigore Ghica Voda 41 A, RO-700487 Iasi, Romania.
Biomacromolecules. 2010 Mar 8;11(3):787-96. doi: 10.1021/bm9014057.
Porous multilayer thin films consisting solely of cross-linked poly(vinyl amine) ((PVAm)(n)) were generated by a selective cross-linking of the PVAm layers in the PVAm/poly(acrylic acid) (PAA) thin films, followed by the removal of PAA. A regular increase in the (PVAm/PAA)(n) multilayer onto silica particles was observed by potentiometric titration when PVAm with a low molar mass (M(w) = 15 000 g mol(-1)) was used in the construction of LbL thin films. The amount of human serum albumin (HSA) loaded on the single-component (PVAm)(5) thin film was approximately 32 mg HSA/g of hybrid when PVAm with a molar mass of 15 000 g mol(-1) was used compared with the single-component film prepared with PVAm with a molar mass of 340 000 g mol(-1) when the amount of HSA loaded was 15.5 mg HSA/g of hybrid. The sorption modalities of HSA and bovine serum albumin (BSA) onto the single-component thin films deposited on silicon wafers as a function of the number of PVAm layers was investigated by atomic force microscopy and contact angle measurements. The decrease in the film roughness and the increase in the film wettability after the loading of both proteins showed the protein was mainly absorbed into the porous (PVAm)(n) thin film.
仅由交联的聚乙烯基胺（（PVAm）（n））组成的多孔多层薄膜是通过[PVAm/聚丙烯酸（PAA）]（n）薄膜中 PVAm 层的选择性交联，然后去除 PAA 来生成的。当在 LbL 薄膜的构建中使用低摩尔质量（M（w）= 15000gmol（-1））的 PVAm 时，通过电位滴定观察到（PVAm/PAA）（n）多层薄膜在二氧化硅颗粒上的规则增加。当使用摩尔质量为 15000gmol（-1）的 PVAm 时，单组分（PVAm）（5）薄膜上负载的人血清白蛋白（HSA）的量约为 32mgHSA/g 混合体，而当使用摩尔质量为 340000gmol（-1）的 PVAm 时，负载的 HSA 量为 15.5mgHSA/g 混合体时，单组分薄膜上负载的 HSA 的量约为 32mgHSA/g 混合体。通过原子力显微镜和接触角测量研究了 HSA 和牛血清白蛋白（BSA）在硅片上沉积的单组分薄膜上的吸附模式作为 PVAm 层数量的函数。在负载两种蛋白质后，薄膜粗糙度的降低和薄膜润湿性的增加表明蛋白质主要被吸收到多孔（PVAm）（n）薄膜中。
