Gómez-Hens Agustina, Fernández-Romero Juan M, Aguilar-Caballos Maria P
Department of Analytical Chemistry, Marie Curie Annex Building, Campus of Rabanales, University of Córdoba, E-14071 Córdoba, Spain.
Comb Chem High Throughput Screen. 2010 May;13(4):309-17. doi: 10.2174/138620710791054240.
An overview of the usefulness of different nanoparticles to improve the features of high throughput separation and individual and multiplexed detection bioassays is presented. Although the development of microarray and microfluidic systems has expanded the capabilities of these high throughput assays, the combined use of NPs and these devices has provided them with new applications in drug discovery, proteomic and genomic studies, and clinical diagnosis. This article reviews the wide application field of magnetic, gold, silver, semiconductor and other nanoparticles in high throughput bioassays. Also, the versatility of the detection systems described shows that NPs are useful alternatives to fluorescent dyes, which are often used in these assays.
本文概述了不同纳米颗粒在改善高通量分离以及个体和多重检测生物测定特性方面的用途。尽管微阵列和微流体系统的发展扩展了这些高通量测定的能力，但纳米颗粒与这些设备的联合使用为它们在药物发现、蛋白质组学和基因组学研究以及临床诊断中提供了新的应用。本文综述了磁性、金、银、半导体和其他纳米颗粒在高通量生物测定中的广泛应用领域。此外，所描述的检测系统的多功能性表明，纳米颗粒是这些测定中常用的荧光染料的有用替代品。
