Rice University, 6100 Main Street, TX 77005, USA.
J Control Release. 2010 Jun 1;144(2):151-8. doi: 10.1016/j.jconrel.2010.02.012. Epub 2010 Feb 13.
A new method of optically guided controlled release was experimentally evaluated with liposomes containing a molecular load and gold nanoparticles (NPs). NPs were exposed to short laser pulses to induce transient vapor bubbles around the NPs, plasmonic nanobubbles, in order to disrupt the liposome and eject its molecular contents. The release efficacy was tuned by varying the lifetime and size of the nanobubble with the fluence of the laser pulse. Optical scattering by nanobubbles correlated to the molecular release and was used to guide the release. The release of two fluorescent proteins from individual liposomes has been directly monitored by fluorescence microscopy, while the generation of the plasmonic nanobubbles was imaged and measured with optical scattering techniques. Plasmonic nanobubble-induced release was found to be a mechanical, nonthermal process that requires a single laser pulse and ejects the liposome contents within a millisecond timescale without damage to the molecular cargo and that can be controlled through the fluence of laser pulse.
一种新的光控释放方法通过含有分子负载和金纳米粒子（NPs）的脂质体进行了实验评估。通过短激光脉冲使 NPs 周围产生瞬态蒸汽泡，即等离子体纳米气泡，以破坏脂质体并排出其分子内容物。通过改变激光脉冲的能量密度来调整纳米气泡的寿命和尺寸，从而调节释放效果。纳米气泡的光散射与分子释放相关，并可用于指导释放。通过荧光显微镜直接监测了两种荧光蛋白从单个脂质体中的释放情况，同时通过光学散射技术对等离子体纳米气泡的产生进行了成像和测量。研究发现，等离子体纳米气泡诱导的释放是一种机械的非热过程，只需要一个激光脉冲，在毫秒级时间内即可将脂质体内容物排出，而不会对分子货物造成损伤，并且可以通过激光脉冲的能量密度来控制。
