Laboratory for Laser Cytotechnologies, A V Lykov Heat and Mass Transfer Institute, 15 Brovka Street, Minsk 220072, Belarus.
Nanotechnology. 2010 Feb 26;21(8):85102. doi: 10.1088/0957-4484/21/8/085102. Epub 2010 Jan 25.
Combining diagnostic and therapeutic processes into one (theranostics) and improving their selectivity to the cellular level may offer significant benefits in various research and disease systems and currently is not supported with efficient methods and agents. We have developed a novel method based on the gold nanoparticle-generated transient photothermal vapor nanobubbles, that we refer to as plasmonic nanobubbles (PNB). After delivery and clusterization of the gold nanoparticles (NP) to the target cells the intracellular PNBs were optically generated and controlled through the laser fluence. The PNB action was tuned in individual living cells from non-invasive high-sensitive imaging at lower fluence to disruption of the cellular membrane at higher fluence. We have achieved non-invasive 50-fold amplification of the optical scattering amplitude with the PNBs (relative to that of NPs), selective mechanical and fast damage to specific cells with bigger PNBs, and optical guidance of the damage through the damage-specific signals of the bubbles. Thus the PNBs acted as tunable theranostic agents at the cellular level and in one process that have supported diagnosis, therapy and guidance of the therapy.
将诊断和治疗过程结合为一体（治疗学），并提高其对细胞水平的选择性，可能会在各种研究和疾病系统中带来重大益处，但目前还没有有效的方法和试剂来支持这一点。我们开发了一种基于金纳米颗粒产生的瞬态光热蒸汽纳米气泡的新方法，我们称之为等离子体纳米气泡（PNB）。金纳米颗粒（NP）被递送到靶细胞并聚集后，通过激光强度来光学产生和控制细胞内的 PNB。通过调节激光强度，PNB 可以在单个活细胞中进行非侵入式的高灵敏度成像，或者在更高的激光强度下破坏细胞膜。我们已经实现了非侵入式的 50 倍光散射幅度放大，与 NPs 相比，通过更大的 PNB 选择性地对特定细胞进行机械和快速破坏，并通过气泡的损伤特异性信号对损伤进行光学引导。因此，PNB 可以作为一种在细胞水平上进行诊断、治疗和治疗指导的可调式治疗学试剂。
