从产气荚膜梭菌C型中纯化β毒素
Purification of beta-toxin from Clostridium perfringens type C.
作者信息
Sakurai J, Duncan C L
出版信息
Infect Immun. 1977 Dec;18(3):741-5. doi: 10.1128/iai.18.3.741-745.1977.
Abstract
Beta-toxin was purified about 340-fold from culture supernatant fluid of Clostridium perfringens type C with a yield of about 24% in terms of biologically active beta-toxin. The purification involved ammonium sulfate fractionation, gel filtration through Sephadex G-100, isoelectrofocusing in a pH 3 to 6 gradient, and immunoaffinity chromatography. The purified beta-toxin gave a single band on polyacrylamide gel electrophoresis.
摘要
从产气荚膜梭菌C型培养上清液中纯化出的β毒素，其比活性提高了约340倍，以具有生物活性的β毒素计，产率约为24%。纯化过程包括硫酸铵分级沉淀、通过Sephadex G - 100进行凝胶过滤、在pH 3至6梯度下进行等电聚焦以及免疫亲和色谱法。纯化后的β毒素在聚丙烯酰胺凝胶电泳上呈现单一条带。
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