高度发光的量子点功能化及其与 IgG 的缀合。

Highly luminescent quantum dots functionalized and their conjugation with IgG.

机构信息

School of Materials Science and Engineering, University of Jinan, 250022 Jinan, PR China.

出版信息

J Colloid Interface Sci. 2010 May 15;345(2):222-7. doi: 10.1016/j.jcis.2010.01.072. Epub 2010 Feb 1.

DOI:10.1016/j.jcis.2010.01.072

PMID:20156624

Abstract

A sol-gel approach including partially removing capping agents, depositing SiO(2) monomers, and growing a SiO(2) shell was developed to generate a bio-compatible functionalization on CdTe quantum dots (QDs). The QDs retained their high photoluminescence (PL) efficiency after coating with a SiO(2) shell (22.5%) by controlling the surface state of the QDs to decrease PL degradation during a sol-gel preparation. Furthermore, the CdTe QDs with a SiO(2) shell were conjugated with IgG using 3-sulfo-N-hydroxysuccinimide or streptavidin-maleimide as linkers. The biotin-streptavidin linker resulted in a high PL efficiency retained (only ~23% lower than the initial value of the QDs), which is crucial for bio-applications.

摘要

采用溶胶-凝胶法，包括部分去除封端剂、沉积 SiO(2)单体和生长 SiO(2)壳，以在 CdTe 量子点 (QD) 上生成生物相容性功能化。通过控制 QD 的表面状态，减少溶胶-凝胶制备过程中 PL 降解，在涂覆 SiO(2)壳后，QD 的光致发光 (PL) 效率仍保持在 22.5%（%）的高水平。此外，将具有 SiO(2)壳的 CdTe QD 与 IgG 用 3-磺基-N-羟基琥珀酰亚胺或链霉亲和素马来酰亚胺作为连接物偶联。生物素-链霉亲和素连接物导致 PL 效率保留较高（仅比 QD 的初始值低约 23%），这对于生物应用至关重要。