Baker J, Metcalf P, Scragg R, Johnson R
Department of Clinical Biochemistry, Green Lane Hospital, Auckland, New Zealand.
Clin Chem. 1991 Apr;37(4):552-6.
We have evaluated Fructosamine Test-Plus, a commercial fructosamine assay based on the reduction of nitro-blue tetrazolium dye in alkaline buffer (Clin Chem 1985;31:1550-4), modified by including a detergent and uricase in the reagent, by changing the concentrations of buffer and dye, and by changing the approach to primary calibration. Specimens were from 2321 participants in a health screening survey in a local workforce. Compared with the original fructosamine method, the Fructosamine Test-Plus method was less affected by protein concentration in the sample and less subject to interference from hyperlipidemia. The changes have also extended the linearity of the assay in the pathological range. However, as a screening method for diabetes mellitus in a population with a disease prevalence of 2.28%, the performance of Fructosamine Test-Plus was similar to the original assay.
我们评估了“果糖胺检测增强版”，这是一种基于在碱性缓冲液中硝基蓝四氮唑染料还原反应的商业果糖胺检测方法（《临床化学》1985年；31:1550 - 4），通过在试剂中加入去污剂和尿酸酶、改变缓冲液和染料的浓度以及改变原始校准方法进行了改良。样本来自当地劳动力健康筛查调查中的2321名参与者。与原始果糖胺方法相比，“果糖胺检测增强版”方法受样本中蛋白质浓度的影响较小，且较少受到高脂血症的干扰。这些改变还扩展了该检测方法在病理范围内的线性。然而，作为一种在疾病患病率为2.28%的人群中筛查糖尿病的方法，“果糖胺检测增强版”的性能与原始检测方法相似。
