Desjarlais F, Comtois R, Beauregard H, Nguyen M, Letellier G
Département de Biochimie, Hôpital Notre-Dame, Montréal, Québec, Canada.
Clin Biochem. 1989 Aug;22(4):329-35. doi: 10.1016/s0009-9120(89)80027-x.
We evaluated a serum fructosamine (glycated serum proteins) assay for efficacy in the diagnosis and follow-up of diabetic patients. A Roche reagent kit, based on nitroblue tetrazolium reduction in alkaline medium, was used in COBAS FARA centrifugal analyzer. We demonstrated that this method is precise, linear and unaffected by serum hemolysis. However, bilirubin affected the test positively and lipemia negatively. Fructosamine (F) correlated positively with total protein (P) (r = 0.809) and albumin (r = 0.746) in a group of 48 non-diabetic individuals. A good correlation was observed between F and glycated hemoglobin from the sera of 514 patients (r = 0.794). A better correlation (r = 0.838) was obtained when F was corrected for P concentration (F/P). Different F and F/P means were calculated only in patients with overt diabetes, compared to normals. Gestational diabetes was associated with a highly significant F increase. However, its low sensitivity (21%) precludes the use of F as an effective screening test for that condition. Nevertheless, because of its simplicity, low cost and rapidity in reflecting changes in the metabolic control of diabetes, F should be considered a valuable test to assess glycemic control in diabetic patients.
我们评估了血清果糖胺（糖化血清蛋白）检测在糖尿病患者诊断及随访中的效果。在COBAS FARA离心分析仪中使用了基于碱性介质中硝基蓝四氮唑还原反应的罗氏试剂试剂盒。我们证明该方法精确、呈线性且不受血清溶血影响。然而，胆红素对检测结果有正向影响，脂血则有负向影响。在一组48名非糖尿病个体中，果糖胺（F）与总蛋白（P）（r = 0.809）和白蛋白（r = 0.746）呈正相关。在514例患者的血清中，观察到F与糖化血红蛋白之间有良好的相关性（r = 0.794）。当F校正P浓度（F/P）时，相关性更好（r = 0.838）。与正常人相比，仅在显性糖尿病患者中计算不同的F和F/P均值。妊娠期糖尿病与F显著升高相关。然而，其低敏感性（21%）使其无法作为该病症的有效筛查试验。尽管如此，由于其操作简便、成本低且能快速反映糖尿病代谢控制的变化，F应被视为评估糖尿病患者血糖控制的一项有价值的检测。
