用于脂蛋白(a)表型分析的改良免疫印迹技术。
Modified immunoblotting technique for phenotyping lipoprotein(a).
作者信息
Huang C M, Kraft H G, Gregg R E
机构信息
National Institutes of Health, Bethesda, MD 20892.
出版信息
Clin Chem. 1991 Apr;37(4):576-8.
We describe a modified immunoblotting method for phenotyping lipoprotein(a) [Lp(a)]. This immunoblotting procedure uses commercially available reagents that have a long shelf life. The method is sensitive and takes only 18 microL of sera. Lp(a) phenotyping can be performed on sera stored at 4 degrees C for as much as a week or at -80 degrees C for as long as a year. In a study of 145 unrelated healthy subjects, we found Lp(a) allelic frequencies of LpF = 1.8%, LpB = 2.6%, LpS1 = 5.1%, LpS2 = 14.8%, LpS3 = 35.9%, LpS4 = 11.6%, and LpO = 28.0%.
我们描述了一种用于脂蛋白(a)[Lp(a)]表型分析的改良免疫印迹法。这种免疫印迹程序使用具有较长保质期的市售试剂。该方法灵敏，仅需18微升血清。Lp(a)表型分析可在4℃储存长达一周或-80℃储存长达一年的血清上进行。在一项对145名无亲缘关系的健康受试者的研究中，我们发现Lp(a)等位基因频率为：LpF = 1.8%，LpB = 2.6%，LpS1 = 5.1%，LpS2 = 14.8%，LpS3 = 35.9%，LpS4 = 11.6%，LpO = 28.0%。
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