Geroldi D, Bellotti V, Buscaglia P, Bonetti G, Gazzaruso C, Caprioli A, Fratino P
Department of Internal Medicine, University of Pavia, IRCCS Policlinico San Matteo, Italy.
Clin Chim Acta. 1993 Nov 30;221(1-2):159-69. doi: 10.1016/0009-8981(93)90030-8.
Apo(a), the specific lipoprotein(a) (Lp(a)) apolipoprotein, is characterized by different isoforms (from 6 to 11 on SDS-PAGE) encoded by a system of autosomal codominant alleles. Electrophoresis on agarose gel displays a better resolving power than SDS-PAGE (a larger number of apo(a) isoforms is detected). The aim of this work was to set up a simple technique that uses a capillary blotting apparatus and a polyvinylidene difluoride membrane for protein transfer. We tested an Italian population sample of 202 healthy subjects (123 men and 79 women) and we detected 22 apo(a) isoforms varying from 280 to 775 kDa. In our sample, 135 subjects (66.5%) had a single-band phenotype, 64 (31.7%) had a double-band phenotype and 3 subjects (1.5%) had no detectable bands ('null' phenotype). This simple and reproducible technique could be applied in the genetic screening of apo(a) polymorphisms and for clinical investigations of the risk of developing cardiovascular diseases.
载脂蛋白(a)是脂蛋白(a)（Lp(a)）的特异性载脂蛋白，其特征在于由常染色体共显性等位基因系统编码的不同异构体（在SDS-PAGE上为6至11种）。琼脂糖凝胶电泳显示出比SDS-PAGE更好的分辨能力（检测到更多数量的载脂蛋白(a)异构体）。这项工作的目的是建立一种使用毛细管印迹装置和聚偏二氟乙烯膜进行蛋白质转移的简单技术。我们检测了202名健康受试者（123名男性和79名女性）的意大利人群样本，检测到22种分子量在280至775 kDa之间的载脂蛋白(a)异构体。在我们的样本中，135名受试者（66.5%）具有单带表型，64名（31.7%）具有双带表型，3名受试者（1.5%）没有可检测到的条带（“无”表型）。这种简单且可重复的技术可应用于载脂蛋白(a)多态性的基因筛查以及心血管疾病发病风险的临床研究。
