Laboratory of Molecular Diagnostics and Immunology, National Institute of Tuberculosis and Lung Diseases, Warsaw, Poland.
Eur J Med Res. 2009 Dec 7;14 Suppl 4(Suppl 4):237-40. doi: 10.1186/2047-783x-14-s4-237.
Free-circulating DNA is present in minute amounts in plasma of healthy individuals, whereas increased levels are found in a number of malignant pathologies including non-small cell lung cancer (NSCLC). The objective of this research was the evaluation of the plasma DNA quantification capacity to distinguish between healthy subjects and non-small cell lung cancer (NSCLC) patients.
Plasma samples were collected prospectively from 16 healthy volunteers and 30 untreated NSCLC patients (I-IIIA). Subsequently, free-circulating DNA extraction and quantitative real-time PCR analysis were performed.
The values of plasma DNA concentration ranged from 0.9 up to 7.0 ng/ml in healthy individuals and from 1.5 up to 50 ng/ml in NSCLC patients before treatment. Cancer group showed several-fold higher mean free-circulating DNA concentration than that present in healthy subjects (mean 12.00 vs. 2.65 ng/ml; P<0.001). A greater variability of plasma DNA concentrations was observed in NSCLC patients than in controls (SD 14.50 vs. 2.02, respectively). The area under the ROC curve was 0.87 (95% CI, 0.744 to 0.954, P<0.001).
Non-small cell lung cancer is associated with elevated levels of cell-free DNA in plasma with respect to healthy controls. Real-time PCR method proved its utility in effective free-circulating DNA detection and quantification.
游离循环 DNA 以微量形式存在于健康个体的血浆中，而在包括非小细胞肺癌（NSCLC）在内的许多恶性病变中发现了水平升高。本研究的目的是评估血浆 DNA 定量能力，以区分健康个体和非小细胞肺癌（NSCLC）患者。
前瞻性收集 16 名健康志愿者和 30 名未经治疗的 NSCLC 患者（I-IIIA）的血浆样本。随后，进行游离循环 DNA 提取和实时定量 PCR 分析。
健康个体的血浆 DNA 浓度值范围为 0.9 至 7.0 ng/ml，而未经治疗的 NSCLC 患者的浓度值范围为 1.5 至 50 ng/ml。与健康受试者相比，癌症组的平均游离循环 DNA 浓度高出数倍（平均值 12.00 与 2.65 ng/ml；P<0.001）。与对照组相比，NSCLC 患者的血浆 DNA 浓度变化更大（SD 分别为 14.50 与 2.02）。ROC 曲线下面积为 0.87（95%CI，0.744 至 0.954，P<0.001）。
与健康对照相比，非小细胞肺癌与血浆中无细胞游离 DNA 水平升高相关。实时 PCR 方法证明了其在有效游离循环 DNA 检测和定量方面的实用性。
