裸鼠的肾毒性血清肾炎：宿主免疫反应的作用
Nephrotoxic serum nephritis in nude rats: the roles of host immune reactions.
作者信息
Sato T, Oite T, Nagase M, Shimizu F
机构信息
Department of Immunology, Niigata University School of Medicine, Japan.
出版信息
Clin Exp Immunol. 1991 Apr;84(1):139-44. doi: 10.1111/j.1365-2249.1991.tb08137.x.
A description is made of renal lesions in rats induced by heterologous (rabbit) nephrotoxic serum with or without subsequent host immune reactions against it and the effects of immune reactions on the course of classical nephrotoxic serum (Masugi) nephritis are discussed. The disease was induced by injecting congenitally athymic ACI nude rats (rnu/rnu) and their normal heterozygous littermates (rnu/+) with rabbit anti-rat glomerular basement membrane (GBM) antiserum. In the autologous phase, rat IgG and immunoglobulins were localized in a linear pattern along capillary walls only in nephritic heterozygous rats. In the indirect plaque-forming cell (PFC) assay against rabbit immunoglobulins in the autologous phase, significantly more PFC could be detected in nephritic heterozygous rats than in nephritic nude rats. The nude and heterozygous rats were essentially the same with respect to the amount of urinary protein, histological change and clinical course. At least in classical nephrotoxic serum nephritis in rats, host immune reactions against GBM bound heterologous nephrotoxic serum were concluded to have no effect on the course of the disease.
本文描述了用异源（兔）肾毒性血清诱导大鼠产生的肾损伤，该过程伴有或不伴有宿主随后针对此血清的免疫反应，并讨论了免疫反应对经典肾毒性血清（马苏吉）肾炎病程的影响。通过给先天性无胸腺的 ACI 裸鼠（rnu/rnu）及其正常杂合子同窝仔鼠（rnu/+）注射兔抗大鼠肾小球基底膜（GBM）抗血清来诱发该疾病。在自身免疫阶段，仅在患肾炎的杂合子大鼠中，大鼠 IgG 和免疫球蛋白沿毛细血管壁呈线性分布。在自身免疫阶段针对兔免疫球蛋白的间接空斑形成细胞（PFC）试验中，与患肾炎的裸鼠相比，在患肾炎的杂合子大鼠中可检测到明显更多的 PFC。就尿蛋白量、组织学变化和临床病程而言，裸鼠和杂合子大鼠基本相同。至少在大鼠的经典肾毒性血清肾炎中，得出结论：宿主针对结合了异源肾毒性血清的 GBM 的免疫反应对疾病病程没有影响。
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