Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
Immune Netw. 2009 Dec;9(6):274-84. doi: 10.4110/in.2009.9.6.274. Epub 2009 Dec 31.
Swiprosin-1 was identified in human CD8+ lymphocytes, mature B cells and non-lymphonoid tissue. We have recently reported that swiprosin-1 is expressed in mast cells and up-regulated in both in vitro and in vivo.
The expression of cytokines and swiprosin-1 were determined by by real time PCR and conventional PCR. Pharmacological inhibitors were treated to investigate potential mechanism of swiprosin-1 in mast cell activation. Actin content was evaluated by confocal microscopy and flow cytometry.
The swiprosin-1 augmented PMA/A23187-induced expression of cytokines and release of histamine. However, knock-down of swiprosin-1 showed only a modest effect on PMA/A23187-induced cytokine expression, suggesting that swiprosin-1 has gain-of-function characteristics. Swiprosin-1 was found in microvilli-like membrane protrusions and highly co-localized with F-actin. Importantly, either disruption of actin by cytochalasin B or inhibition of PI3 kinase, an enzyme involved in actin remodeling, by wortmannin blocked cytokine expression only in swiprosin-1-overexpressing cells.
These results suggest that swiprosin-1 modulates mast cell activation potentially through actin regulation.
Swiprosin-1 最初在人类 CD8+ 淋巴细胞、成熟 B 细胞和非淋巴组织中被发现。我们最近的研究表明，Swiprosin-1 在肥大细胞中表达，并在体外和体内均被上调。
通过实时 PCR 和常规 PCR 测定细胞因子和 Swiprosin-1 的表达。用药理学抑制剂处理以研究 Swiprosin-1 在肥大细胞活化中的潜在机制。通过共聚焦显微镜和流式细胞术评估肌动蛋白含量。
Swiprosin-1 增强了 PMA/A23187 诱导的细胞因子表达和组胺释放。然而，Swiprosin-1 的敲低对 PMA/A23187 诱导的细胞因子表达仅显示出适度的影响，这表明 Swiprosin-1 具有获得性功能特征。Swiprosin-1 存在于微绒毛样膜突起中，并与 F-肌动蛋白高度共定位。重要的是，细胞松弛素 B 破坏肌动蛋白或wortmannin 抑制参与肌动蛋白重塑的 PI3 激酶，仅在 Swiprosin-1 过表达细胞中阻断细胞因子表达。
这些结果表明，Swiprosin-1 通过调节肌动蛋白来调节肥大细胞的活化。
