发根农杆菌介导的绒毛莸（Hypericum tomentosum L）和四翅滨藜（Hypericum tetrapterum Fries）的遗传转化。
Agrobacterium rhizogenes-mediated transformation of Hypericum tomentosum L and Hypericum tetrapterum Fries.
机构信息
P. J. Safirik University, Faculty of Science, Institute of Biology and Ecology, Mánesova 23, 041 54 KoSice, Slovakia.
出版信息
Z Naturforsch C J Biosci. 2009 Nov-Dec;64(11-12):864-8. doi: 10.1515/znc-2009-11-1218.
This is the first evidence on successful Agrobacterium rhizogenes-mediated genetic transformation of two species from the genus Hypericum, H. tomentosum L. and H. tetrapterum Fries. Hairy root cultures were induced from root segments of both Hypericum species by two agropine wild-type strains of A. rhizogenes, ATCC 15834 and A4. The transgenic character of the hairy root cultures was proved by PCR amplification of the rolABCD genes. In some H. tetrapterum transgenic lines aux genes were detected as well.
这是首次成功将根癌农杆菌介导的遗传转化应用于金丝桃属（Hypericum）两个物种的证据，这两个物种分别是贯叶连翘（H. tomentosum L.）和长花柱金丝桃（H. tetrapterum Fries）。通过使用根癌农杆菌的两个野生型菌株 ATCC 15834 和 A4，从这两个金丝桃物种的根段中诱导出了发根培养物。通过对 rolABCD 基因的 PCR 扩增证明了发根培养物的转基因特性。在一些长花柱金丝桃的转基因系中也检测到了 aux 基因。
Zotero 插件