Laboratory of Plant Biotechnology, School of Life Sciences, Sambalpur University, Sambalpur-768019, Orissa, India.
Z Naturforsch C J Biosci. 2009 Nov-Dec;64(11-12):882-90. doi: 10.1515/znc-2009-11-1221.
Genetic linkage analysis of 151 restriction fragment length polymorphism (RFLP) loci, that included eight new loci, detected by the six probes in the present study, and four trait loci including seed colour, leaf pubescence, resistance to white rust caused by Albugo candida race-2 (AC-2) and race-7 (AC-7) employing the MAPMAKER/EXP 3.0 programme led to the development of 10 linkage groups (LGs) spanning over 44.4 centiMorgan (cM) to 130.4 cM containing 9 to 22 loci and two short LGs with two or three marker loci in Brassica rapa. The enriched map covers 993.1 cM of B. rapa genome with an average marker interval of 6.41. Eight new RFLP loci occupied new map positions on five linkage groups, LG 2, 3, 6, 8 and 9. Addition of these RFLP loci led to appreciable changes in the corresponding linkage groups and resulted in an increase of the total map length by 102.8 cM and of the marker interval by 0.35 cM. Interval mapping by using the computer programme MAPMAKER/ QTL 1.1 for scanning the genetic map led to the detection of one major quantitative trait locus (QTL) in LG 4 and one minor QTL in LG 8 governing resistance to AC-7. Both QTLs contributed 7.89 to the interaction phenotype (IP) score with 96.3% genetic variation. The multi-locus model suggested additive gene action with 96.8% genetic variation.
采用本研究中的 6 个探针检测到的 151 个限制片段长度多态性（RFLP）位点的遗传连锁分析，包括 8 个新位点，以及利用 MAPMAKER/EXP 3.0 程序检测到的包括种皮颜色、叶片柔毛、对由 Albugo candida race-2（AC-2）和 race-7（AC-7）引起的白锈病的抗性在内的 4 个性状位点，导致了 10 个连锁群（LG）的发展，这些 LG 跨越 44.4 厘摩（cM）到 130.4 cM，包含 9 到 22 个位点，以及在白菜中具有两个或三个标记位点的两个短 LG。这个丰富的图谱涵盖了白菜基因组的 993.1 cM，平均标记间隔为 6.41。8 个新的 RFLP 位点占据了五个连锁群，即 LG 2、3、6、8 和 9 上的新图谱位置。这些 RFLP 位点的加入导致了相应的连锁群发生了显著的变化，使得图谱总长度增加了 102.8 cM，标记间隔增加了 0.35 cM。利用计算机程序 MAPMAKER/QTL 1.1 对遗传图谱进行区间作图，导致在 LG 4 中检测到一个主数量性状位点（QTL）和在 LG 8 中检测到一个控制对 AC-7 的抗性的小 QTL。这两个 QTL 对交互表型（IP）评分的贡献分别为 7.89，遗传变异率为 96.3%。多基因座模型表明，加性基因作用的遗传变异率为 96.8%。
