Molecular Genetics and Genomics Lab, Department of Horticulture, Chungnam National University, Gung-Dong, Yuseong-Gu, Daejeon 305 764, Republic of Korea.
Genome. 2010 Nov;53(11):939-47. doi: 10.1139/G10-054.
We constructed a high-density Brassica rapa integrated linkage map by combining a reference genetic map of 78 doubled haploid lines derived from Chiifu-401-42 × Kenshin (CKDH) and a new map of 190 F2 lines derived from Chiifu-401-42 × rapid cycling B. rapa (CRF2). The integrated map contains 1017 markers and covers 1262.0 cM of the B. rapa genome, with an average interlocus distance of 1.24 cM. High similarity of marker order and position was observed among the linkage groups of the maps with few short-distance inversions. In total, 155 simple sequence repeat (SSR) markers, anchored to 102 new bacterial artificial chromosomes (BACs) and 146 intron polymorphic (IP) markers were mapped in the integrated map, which would be helpful to align the sequenced BACs in the ongoing multinational Brassica rapa Genome Sequencing Project (BrGSP). Further, comparison of the B. rapa consensus map with the 10 B. juncea A-genome linkage groups by using 98 common IP markers showed high-degree colinearity between the A-genome linkage groups, except for few markers showing inversion or translocation. Suggesting that chromosomes are highly conserved between these Brassica species, although they evolved independently after divergence. The sequence information coming out of BrGSP would be useful for B. juncea breeding. and the identified Arabidopsis chromosomal blocks and known quantitative trait loci (QTL) information of B. juncea could be applied to improve other Brassica crops including B. rapa.
我们构建了一个高密度的芸薹属作物连锁图谱，该图谱综合了来自 Chiifu-401-42×Kenshin（CKDH）的 78 个双单倍体系的参考遗传图谱和来自 Chiifu-401-42×快速循环芸薹属作物（CRF2）的 190 个 F2 系的新图谱。该综合图谱包含 1017 个标记，覆盖了芸薹属作物基因组的 1262.0cM，标记间平均距离为 1.24cM。图谱的连锁群之间标记顺序和位置高度相似，只有少数短距离倒位。总共将 155 个简单序列重复（SSR）标记、锚定到 102 个新的细菌人工染色体（BAC）和 146 个内含子多态性（IP）标记映射到综合图谱中，这将有助于对齐正在进行的多国芸薹属作物基因组测序计划（BrGSP）中测序的 BAC。此外，通过使用 98 个共同的 IP 标记，将芸薹属作物共识图谱与 10 个芸薹属作物 A 基因组连锁群进行比较，结果显示 A 基因组连锁群之间具有高度的共线性，除了少数标记显示倒位或易位。这表明这些芸薹属物种之间的染色体高度保守，尽管它们在分化后独立进化。BrGSP 产生的序列信息将对芸薹属作物的育种有用，并且鉴定出的拟南芥染色体块和已知的芸薹属作物数量性状位点（QTL）信息可应用于改良包括芸薹属作物在内的其他芸薹属作物。
