Guo Ying, Shi Dejin, Li Wensheng, Liang Chaofeng, Wang Hui, Ye Zhuopeng, Hu Liping, Li Yan
Department of Neurosurgery, 3rd Affiliated Hospital, Basic Medical College, Sun Yat-Sen University, Guangzhou, China.
Br J Neurosurg. 2010 Feb;24(1):62-8. doi: 10.3109/02688690903506077.
In adult mammalian brain, microvessels may produce a special microenvironment and then influence the characteristics of neural stem cells (NSCs). This work aims to compare the effects of cerebral microvascular endothelial cells (CMECs) and vascular endothelial growth factor (VEGF) on the behaviors of NSCs. An in vitro model with co-cultured CMECs and NSCs was established by using transwell co-culture system. The expression of nestin and NF in the early stage of the co-culture, and NF in the later stage were explored by immunostaining. Results demonstrate that both CMECs and VEGF stimulate the proliferation of NSCs and inhibit the differentiation. After removing CMECs or VEGF from the co-culture system, a further culture in medium for the differentiation of NSCs was performed. The percentage of NF-positive cells in the CMECs co-culture group is the highest, suggesting the improved neuronal differentiation of NSCs by CMECs.
在成年哺乳动物大脑中，微血管可能产生特殊的微环境，进而影响神经干细胞（NSCs）的特性。本研究旨在比较脑微血管内皮细胞（CMECs）和血管内皮生长因子（VEGF）对神经干细胞行为的影响。采用Transwell共培养系统建立了CMECs与神经干细胞共培养的体外模型。通过免疫染色检测共培养早期巢蛋白和神经丝蛋白（NF）的表达以及后期NF的表达。结果表明，CMECs和VEGF均能刺激神经干细胞增殖并抑制其分化。从共培养体系中去除CMECs或VEGF后，在用于神经干细胞分化的培养基中进一步培养。CMECs共培养组中NF阳性细胞的百分比最高，表明CMECs促进了神经干细胞向神经元的分化。
