Department of Physiology, Medical School, Institute for Medical Sciences, Chonbuk National University, JeonJu 561-180, Republic of Korea.
Toxicol In Vitro. 2010 Jun;24(4):1183-9. doi: 10.1016/j.tiv.2010.02.013. Epub 2010 Feb 14.
Propyl gallate (PG) as a synthetic antioxidant exerts a variety of effects on tissue and cell functions. Here, we evaluated the effects of PG on the growth and death of endothelial cells (ECs), especially calf pulmonary artery endothelial cells (CPAEC) in relation to reactive oxygen species (ROS) and glutathione (GSH). PG dose-dependently inhibited the growth of CPAEC and human umbilical vein endothelial cells (HUVEC) at 24h. PG induced cell death in CPAEC, which was accompanied by the loss of mitochondrial membrane potential (MMP; DeltaPsi(m)). PG generally increased ROS level in CPAEC but not in HUVEC. PG also dose-dependently increased GSH depleted cells in both ECs. The treatment with antioxidant of N-acetyl-cysteine (NAC) or ascorbate acid (AA) prevented CPAEC growth inhibition and death by PG, which was accompanied by the attenuation of GSH depletion but not by the reduction of ROS level. In conclusion, PG induced growth inhibition and death of ECs, especially CPAEC via GSH depletion.
没食子酸丙酯（PG）作为一种合成抗氧化剂，对组织和细胞功能有多种影响。在这里，我们评估了 PG 对内皮细胞（EC）生长和死亡的影响，特别是小牛肺动脉内皮细胞（CPAEC），并与活性氧（ROS）和谷胱甘肽（GSH）有关。PG 以剂量依赖的方式在 24 小时内抑制 CPAEC 和人脐静脉内皮细胞（HUVEC）的生长。PG 诱导 CPAEC 细胞死亡，伴随着线粒体膜电位（MMP；DeltaPsi(m)）的丧失。PG 通常会增加 CPAEC 中的 ROS 水平，但不会增加 HUVEC 中的 ROS 水平。PG 还以剂量依赖的方式增加了两种 EC 中的 GSH 耗竭细胞。抗氧化剂 N-乙酰半胱氨酸（NAC）或抗坏血酸（AA）的处理可防止 PG 引起的 CPAEC 生长抑制和死亡，这伴随着 GSH 耗竭的减弱，但不会降低 ROS 水平。总之，PG 通过 GSH 耗竭诱导 EC，特别是 CPAEC 的生长抑制和死亡。
Zotero 插件