Okabe H, DuBuy J, Hatanaka M, Gilden R V
Intervirology. 1978;9(4):253-60. doi: 10.1159/000148943.
Differences in the multiplicity of proviral sequences of two feline endogenous viruses, RD-114 and FeLV, in homologous and heterologous host DNA were examined by viral cDNA hybridization using cellular DNA fractionated with respect to reiteration frequency. The endogenous proviral DNAs in cat cells were fractionated predominantly with intermediate-repeated sequences, while RD-114 proviral DNA in virus-infected human cells was fractionated together with unique-sequence DNA. These differences were consistent with the results of kinetic analysis using unfractionated DNA.
利用根据重复频率分级分离的细胞DNA，通过病毒cDNA杂交，检测了两种猫内源性病毒RD - 114和FeLV在同源和异源宿主DNA中前病毒序列拷贝数的差异。猫细胞中的内源性前病毒DNA主要与中等重复序列一起分级分离，而病毒感染的人细胞中的RD - 114前病毒DNA则与单拷贝序列DNA一起分级分离。这些差异与使用未分级分离的DNA进行动力学分析的结果一致。
