Max Planck-Institute for Metals Research, Department of New Materials and Biosystems, Stuttgart, Germany.
Biophys J. 2010 Feb 17;98(4):543-51. doi: 10.1016/j.bpj.2009.11.001.
Spatial patterning of biochemical cues on the micro- and nanometer scale controls numerous cellular processes such as spreading, adhesion, migration, and proliferation. Using force microscopy we show that the lateral spacing of individual integrin receptor-ligand bonds determines the strength of cell adhesion. For spacings > or = 90 nm, focal contact formation was inhibited and the detachment forces as well as the stiffness of the cell body were significantly decreased compared to spacings < or = 50 nm. Analyzing cell detachment at the subcellular level revealed that rupture forces of focal contacts increase with loading rate as predicted by a theoretical model for adhesion clusters. Furthermore, we show that the weak link between the intra- and extracellular space is at the intracellular side of a focal contact. Our results show that cells can amplify small differences in adhesive cues to large differences in cell adhesion strength.
在微观和纳米尺度上，生化线索的空间模式控制着许多细胞过程，如扩散、黏附、迁移和增殖。使用力显微镜，我们发现单个整合素受体-配体键的横向间距决定了细胞黏附的强度。对于间距>或=90nm，与间距<或=50nm 相比，焦点接触的形成受到抑制，细胞体的分离力以及刚性显著降低。在亚细胞水平分析细胞分离发现，根据黏附簇的理论模型预测，焦点接触的破裂力随加载速率增加而增加。此外，我们还表明，细胞内和细胞外空间之间的薄弱环节位于焦点接触的细胞内一侧。我们的结果表明，细胞可以将黏附线索的微小差异放大为细胞黏附强度的巨大差异。
