Müller Daniel J, Helenius Jonne, Alsteens David, Dufrêne Yves F
Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
Nat Chem Biol. 2009 Jun;5(6):383-90. doi: 10.1038/nchembio.181.
Biological processes rely on molecular interactions that can be directly measured using force spectroscopy techniques. Here we review how atomic force microscopy can be applied to force probe surfaces of living cells to single-molecule resolution. Such probing of individual interactions can be used to map cell surface receptors, and to assay the receptors' functional states, binding kinetics and landscapes. This information provides unique insight into how cells structurally and functionally modulate the molecules of their surfaces to interact with the cellular environment.
生物过程依赖于分子间相互作用，而这些相互作用可以使用力谱技术直接测量。在此，我们综述了原子力显微镜如何应用于对活细胞表面进行力探测，以达到单分子分辨率。对单个相互作用的这种探测可用于绘制细胞表面受体图谱，并分析受体的功能状态、结合动力学和构象。这些信息为细胞如何在结构和功能上调节其表面分子以与细胞环境相互作用提供了独特的见解。
