Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital, Sagamihara, Japan; Division of Third Department of Internal Medicine, Oita University Faculty of Medicine, Yufu, Oita 879-5593, Japan.
J Allergy Clin Immunol. 2010 Feb;125(2):483-489.e3. doi: 10.1016/j.jaci.2009.10.074.
CD203c is a basophil cell surface marker used to diagnose and monitor various allergic diseases, but its relationship to asthma is not clear.
We determined whether CD203c expression levels are associated with stable and exacerbated asthma.
We used flow cytometry to compare spontaneous expression levels of surface markers on basophils from patients with stable or exacerbated asthma and from healthy subjects. Longitudinal changes in these expression levels were measured after basophil stimulation by IgE-dependent or IgE-independent mechanisms and compared with patients' asthma status.
Spontaneous expression levels of CD203c were significantly higher on basophils from patients with asthma exacerbation than patients with stable asthma or healthy subjects. In contrast, no differences in spontaneous expression levels of CD63 or CD69 were observed among the 3 groups. Anti-IgE-induced expression of CD203c significantly increased in basophils during asthma exacerbation (P = .005). Low concentrations of Dermatophagoides pteronyssinus or IL-3 induced higher expression levels of CD203c during asthma exacerbation than during clinical improvement; induction of CD203c expression by these antigens therefore correlates with asthma control. In the patients with clinical improvement, there was a correlation between spontaneous CD203c expression levels and the percent predicted values of FEV(1) (r = -0.761; P = .022).
Asthma exacerbation was accompanied by increased expression of CD203c on basophils that decreased significantly during remission. Basophil expression levels of CD203c might therefore be used to monitor asthma in patients.
CD203c 是一种嗜碱性粒细胞表面标志物，用于诊断和监测各种过敏性疾病，但它与哮喘的关系尚不清楚。
我们旨在确定 CD203c 的表达水平是否与稳定期和加重期哮喘相关。
我们使用流式细胞术比较了稳定期和加重期哮喘患者以及健康对照者嗜碱性粒细胞表面标志物的自发表达水平。通过 IgE 依赖性或 IgE 非依赖性机制刺激嗜碱性粒细胞后，测量这些表达水平的纵向变化，并与患者的哮喘状态进行比较。
与稳定期哮喘患者或健康对照者相比，哮喘加重患者的嗜碱性粒细胞自发表达 CD203c 的水平显著更高。相比之下，3 组患者嗜碱性粒细胞的自发表达 CD63 或 CD69 水平无差异。在哮喘加重期间，抗 IgE 诱导的 CD203c 表达显著增加（P =.005）。在哮喘加重期间，低浓度的屋尘螨或 IL-3 诱导 CD203c 表达水平高于临床改善期间，因此这些抗原诱导 CD203c 表达与哮喘控制相关。在临床改善的患者中，自发的 CD203c 表达水平与 FEV1 预计值的百分比之间存在相关性（r = -0.761；P =.022）。
哮喘加重时嗜碱性粒细胞上的 CD203c 表达增加，在缓解期显著下降。因此，嗜碱性粒细胞 CD203c 的表达水平可能用于监测哮喘患者的病情。
