Xie Hua, Chen Liping, Zhang Huiyun, Wang Junling, Zang Yanyan, Zhan Mengmeng, Gu Fangqiu, Wang Shunlan, He Shaoheng
The PLA Center of Respiratory and Allergic Disease Diagnosing Management, General Hospital of Northern Theater Command, Shenyang, China.
Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.
Front Mol Biosci. 2022 Aug 11;9:907092. doi: 10.3389/fmolb.2022.907092. eCollection 2022.
Altered basophil identification markers have been discovered to associate with allergic asthma (AA) in recent years. However, little is known about the expression of basophil markers in blood granulocytes. To parallel test blood basophils in peripheral blood mononuclear cell (PBMC) and granulocyte populations of patients with AA and AA combined with allergic rhinitis (ARA) The expressions of surface molecules were determined flow cytometry. CD123 expressing cells in blood were isolated using a cell sorting technique, and mouse AA models were employed for study. The numbers of CD123HLA-DR cells in the granulocytes of AA and ARA patients markedly increased. However, only 49.7% of CD123HLA-DR cells in granulocytes and 99.0% of CD123HLA-DR cells in PBMCs were basophils. Almost all CD123HLA-DR cells expressed CD63 regardless in granulocytes or PBMC. The numbers of CD63, Fc epsilon receptor I (FcεRI), and CD203c expressing cells markedly enhanced in CD123HLA-DR granulocytes of AA and ARA patients. Mean fluorescence intensity (MFI) of CD63 and CD203c expressions on CD123HLA-DR PBMC and granulocytes of AA and ARA patients dramatically elevated. House dust mite extract (HDME) and wild allergen extract (ASWE) enhanced the numbers of CD63CD123HLA-DR granulocytes and PBMC and the MFI of CD203c expression on CD123HLA-DR granulocyte of AA and ARA patients. Histamine, tryptase, and PGD2 enhanced proportions of CD123 KU812 cells. ASWE- and HDME-induced AA mice showed upregulated CD63 expression on basophils. In conclusion, upregulated expressions of CD123, CD203c, CD63, and FcεRIα in PBMC and granulocytes of patients with AA and ARA suggest that CD123HLA-DR cells may contribute to the development of AA and ARA.
近年来发现嗜碱性粒细胞识别标志物的改变与过敏性哮喘（AA）相关。然而，关于嗜碱性粒细胞标志物在血液粒细胞中的表达情况却知之甚少。为了同时检测AA患者以及AA合并过敏性鼻炎（ARA）患者外周血单个核细胞（PBMC）和粒细胞群体中的血液嗜碱性粒细胞，采用流式细胞术测定表面分子的表达。利用细胞分选技术分离血液中表达CD123的细胞，并采用小鼠AA模型进行研究。AA和ARA患者粒细胞中CD123 HLA - DR细胞数量显著增加。然而，粒细胞中只有49.7%的CD123 HLA - DR细胞以及PBMC中99.0%的CD123 HLA - DR细胞为嗜碱性粒细胞。几乎所有CD123 HLA - DR细胞，无论在粒细胞还是PBMC中，均表达CD63。AA和ARA患者CD123 HLA - DR粒细胞中表达CD63、Fcε受体I（FcεRI）和CD203c的细胞数量显著增加。AA和ARA患者CD123 HLA - DR PBMC和粒细胞上CD63和CD203c表达的平均荧光强度（MFI）显著升高。屋尘螨提取物（HDME）和野生变应原提取物（ASWE）增加了AA和ARA患者CD63 CD123 HLA - DR粒细胞和PBMC的数量以及CD123 HLA - DR粒细胞上CD203c表达的MFI。组胺、类胰蛋白酶和前列腺素D2增加了CD123⁺ KU812细胞的比例。ASWE和HDME诱导的AA小鼠嗜碱性粒细胞上CD63表达上调。总之，AA和ARA患者PBMC和粒细胞中CD123、CD203c、CD63和FcεRIα表达上调表明CD123 HLA - DR细胞可能参与AA和ARA的发病过程。
