利用米曲霉 RIB40 的转化体在液体表面固定化（LSI）系统中生产木聚糖酶。

Production of Xylanase with a transformant of Aspergillus oryzae RIB40 in a Liquid-Surface Immobilization (LSI) System.

机构信息

Genome Biotechnology Laboratory, Kanazawa Institute of Technology, 3-1 Yatsukaho, Hakusan, Ishikawa 924-0838, Japan.

出版信息

J Biosci Bioeng. 2010 Mar;109(3):224-6. doi: 10.1016/j.jbiosc.2009.08.006. Epub 2009 Sep 9.

DOI:10.1016/j.jbiosc.2009.08.006

PMID:20159567

Abstract

Xylanase production by a XynF1 (33 kDa)-transformant of Aspergillus oryzae RIB40 was compared between submerged cultivation (SmC) and liquid-surface immobilization (LSI) systems. While the accumulation of xylanase in the SmC decreased by prolonged incubation, LSI system enabled the continuation of xylanase production to afford 4.5-fold xylanase production compared with the SmC system.

摘要

木聚糖酶生产由木聚糖酶 1 (33 kDa)转化子的米曲霉 RIB40 进行了比较之间的浸没式培养（SmC）和液体表面固定化（LSI）系统。而在 SmC 中的木聚糖酶积累通过延长孵育减少，LSI 系统使木聚糖酶生产的延续能够提供 4.5 倍的木聚糖酶生产比 SmC 系统。