Raben N, Barbetti F, Cama A, Lesniak M A, Lillioja S, Zimmet P, Serjeantson S W, Taylor S I, Roth J
Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health, Bethesda, MD 20892.
Diabetes. 1991 Jan;40(1):118-22. doi: 10.2337/diab.40.1.118.
The nucleotide sequence of the insulin gene was determined in American Pima Indians and Micronesian Nauruans, two populations in whom the prevalence of non-insulin-dependent (type II) diabetes mellitus is the highest in the world. The insulin gene was amplified by the polymerase chain reaction to generate single-stranded DNA suitable for direct sequencing. The nucleotide sequences of the coding and adjacent regions of the insulin gene in six Pima Indians and two Nauruans with type II diabetes were identical to previously published insulin gene sequences of nondiabetic subjects.
对美国皮马印第安人和密克罗尼西亚瑙鲁人这两个人群的胰岛素基因核苷酸序列进行了测定，这两个人群中非胰岛素依赖型（II型）糖尿病的患病率在世界上是最高的。通过聚合酶链反应扩增胰岛素基因以生成适合直接测序的单链DNA。六名患II型糖尿病的皮马印第安人和两名瑙鲁人的胰岛素基因编码区及相邻区域的核苷酸序列与先前发表的非糖尿病受试者的胰岛素基因序列相同。
