Zhang Hui, Xue Zhong-lin, Qi An-min, Li Sin, Ye Jian-dong
Department of Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Feb;30(2):278-83.
To investigate the antitumor effect of calcium phosphate cement incorporated with doxorubicin microspheres.
The absorbance at 490 nm of SaoS-2 cells cultured for 5 days in the media containing the extract of the cement incorporating doxorubicin microspheres was measured using Cell Counting Kit-8. SaoS-2 cells were adjusted to the density of 2x10(7) ml(-1) and injected into the left buttock of nude mouse in the volume of 0.2 ml. The cell suspension (0.1 ml) mixed with an equal volume of the cement extract were injected into the right buttock and on the back of the bilateral ears of nude mice. At 12 days after the cell injection, the tumor tissues were obtained and weighed to calculate the tumor inhibition rate, and the pathological samples were observed with HE staining.
The extract of the bone cement containing doxorubicin microspheres showed inhibitory effects on the tumor growth in a dose-dependent manner. The tumor inhibition rate reached 61.0% in high-dose group. Tumor necrosis was found in high dose group, but virtually absent in low-dose group.
CPC containing doxorubicin PLGA microspheres can inhibit tumor cell growth both in vitro and in vivo.
研究含阿霉素微球的磷酸钙骨水泥的抗肿瘤作用。
使用细胞计数试剂盒-8测定在含掺有阿霉素微球的骨水泥提取物的培养基中培养5天的SaoS-2细胞在490nm处的吸光度。将SaoS-2细胞调整至密度为2×10⁷ml⁻¹，并以0.2ml的体积注射到裸鼠的左臀部。将与等体积骨水泥提取物混合的细胞悬液（0.1ml）注射到裸鼠的右臀部和双侧耳背。细胞注射后12天，获取肿瘤组织并称重以计算肿瘤抑制率，并用苏木精-伊红染色观察病理样本。
含阿霉素微球的骨水泥提取物对肿瘤生长具有剂量依赖性抑制作用。高剂量组肿瘤抑制率达到61.0%。高剂量组发现肿瘤坏死，而低剂量组几乎没有。
含阿霉素聚乳酸-羟基乙酸共聚物微球的磷酸钙骨水泥在体外和体内均可抑制肿瘤细胞生长。
