Xiang Guo-an, Chen Kai-yun, Wang Han-ning, Xiao Jin-feng
Department of General Surgery, Second People's Hospital of Guangdong Province, Guangzhou 510317, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Feb;30(2):292-4.
To investigate the effect of iodine-125 implantation on immune cell subsets and cytokine production in patients undergoing hepatocellular carcinoma (HCC) resection.
Sixty-eight patients with resectable HCC were randomly divided into intrahepatic iodine-125 embedding group and control group. The percentages of T lymphocyte phenotypes (CD3(+), CD4(+), CD8(+)), NK cells, and the plasma concentrations of IL-4, IL-10, IL-12 and IFN-gamma of the patients were determined with flow cytometry and ELISA, respectively.
In the control group, the postoperative CD3(+) and CD4(+) immunocytes were (39.38-/+6.98)% and (24.34-/+3.18)%, significantly lower than the preoperative levels [(62.58-/+8.67)% and (30.63-/+4.19)%, respectively, P<0.05)]. The postoperative concentrations of IL-4 and IL-10 were (29.83-/+7.99) and (87.54-/+2.89) ng/L, significantly higher than the preoperative levels (10.35-/+8.76 and 64.25-/+2.54 ng/L, respectively, P<0.05). In the treatment group, the percentages of the immunocytes and cytokine concentrations underwent no significant changes after the operation, but postoperative IL-12 (89.46-/+11.43 ng/L) and IFN-gamma (47.78-/+5.45 ng/L) levels were significantly higher than the preoperative levels (36.13-/+9.16 and 7.14-/+2.17 ng/L, respectively, P<0.05). Significant differences were found between the two groups in the postoperative CD3(+) and CD4(+) immunocytes and IL-4, IL-10, IL-12 and IFN-gamma levels.
Iodine-125 implantation can strongly stimulates the anti-tumor immune response in HCC patients by increasing CD3(+) and CD4(+) immunocytes and promoting Th2/Th1 deviation.
探讨碘-125植入对肝细胞癌（HCC）切除患者免疫细胞亚群及细胞因子产生的影响。
将68例可切除性HCC患者随机分为肝内碘-125植入组和对照组。分别采用流式细胞术和酶联免疫吸附测定法测定患者T淋巴细胞表型（CD3(+)、CD4(+)、CD8(+)）、NK细胞的百分比以及血浆中IL-4、IL-10、IL-12和IFN-γ的浓度。
对照组术后CD3(+)和CD4(+)免疫细胞分别为(39.38±6.98)%和(24.34±3.18)%，显著低于术前水平[(分别为(62.58±8.67)%和(30.63±4.19)%，P<0.05)]。术后IL-4和IL-10浓度分别为(29.83±7.99)和(87.54±2.89) ng/L，显著高于术前水平(分别为10.35±8.76和64.25±2.54 ng/L，P<0.05)。治疗组术后免疫细胞百分比和细胞因子浓度无明显变化，但术后IL-12(89.46±11.43 ng/L)和IFN-γ(47.78±5.45 ng/L)水平显著高于术前水平(分别为36.13±9.16和7.14±2.17 ng/L，P<0.05)。两组术后CD3(+)和CD4(+)免疫细胞以及IL-4、IL-10、IL-12和IFN-γ水平存在显著差异。
碘-125植入可通过增加CD3(+)和CD4(+)免疫细胞并促进Th2/Th1偏移，强烈刺激HCC患者的抗肿瘤免疫反应。
