Gruntar Igor, Ocepek Matjaz, Avbersek Jana, Mićunović Jasna, Pate Mateja
University of Ljubljana Institute of Microbiology and Parasitology, Veterinary Faculty Gerbiceva 60 1000 Ljubljana Slovenia.
Acta Vet Hung. 2010 Mar;58(1):19-28. doi: 10.1556/AVet.58.2010.1.2.
Campylobacter jejuni and C. coli have recently become the most frequent cause of bacterial foodborne enteric infection in most industrialised countries. Consumption and handling of undercooked contaminated poultry meat was identified as an important risk factor for human campylobacteriosis. The aim of this study was to ascertain the genetic diversity of C. jejuni and C. coli strains isolated from poultry in Slovenia. A total of 68 isolates (42 C. jejuni , 26 C. coli ) from faeces (n = 48), meat (n = 15) and skin/carcasses (n = 5) of chicken (n = 60) and turkey samples (n = 5) were analysed by pulsed-field gel electrophoresis. Sma I macrorestriction discriminated between C. jejuni and C. coli isolates. C. jejuni isolates exhibited a higher degree of diversity compared to C. coli isolates. In the C. jejuni group, a number of small clusters were apparent, while C. coli strains formed less but larger clusters. Additional Kpn I digestion of selected isolates resulted in poor subtyping. Strains with identical or very similar profiles were found on different farms, either in the same or different regions and time periods. Some of the clones indicated possible cross-contamination at slaughterhouses.
空肠弯曲菌和结肠弯曲菌最近已成为大多数工业化国家细菌性食源性肠道感染的最常见病因。食用和处理未煮熟的受污染禽肉被确定为人类弯曲菌病的一个重要风险因素。本研究的目的是确定从斯洛文尼亚家禽中分离出的空肠弯曲菌和结肠弯曲菌菌株的遗传多样性。通过脉冲场凝胶电泳分析了从60只鸡和5只火鸡的粪便（n = 48）、肉（n = 15）和皮肤/胴体（n = 5）中总共分离出的68株菌株（42株空肠弯曲菌，26株结肠弯曲菌）。Sma I酶切可区分空肠弯曲菌和结肠弯曲菌分离株。与结肠弯曲菌分离株相比，空肠弯曲菌分离株表现出更高的多样性。在空肠弯曲菌组中，出现了许多小簇，而结肠弯曲菌菌株形成的簇较少但较大。对选定分离株进行额外的Kpn I酶切导致亚型分型效果不佳。在不同农场，无论是在同一地区还是不同地区以及不同时间段，都发现了具有相同或非常相似图谱的菌株。一些克隆表明屠宰场可能存在交叉污染。
