Department of Orthodontics, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Eur J Orthod. 2010 Apr;32(2):131-9. doi: 10.1093/ejo/cjp078. Epub 2010 Feb 16.
It has previously been reported that low-energy laser irradiation stimulated the velocity of tooth movement via the receptor activator of nuclear factor kappa B (RANK)/RANK ligand and the macrophage colony-stimulating factor/its receptor (c-Fms) systems. Matrix metalloproteinase (MMP)-9, cathepsin K, and alpha(v) beta(3) [alpha(v)beta3] integrin are essential for osteoclastogenesis; therefore, the present study was designed to examine the effects of low-energy laser irradiation on the expression of MMP-9, cathepsin K, and alpha(v)beta3 integrin during experimental tooth movement. Fifty male, 6-week-old Wistar strain rats were used in the experiment. A total force of 10g was applied to the rat molars to induce tooth movement. A Ga-Al-As diode laser was used to irradiate the area around the moving tooth and, after 7 days, the amount of tooth movement was measured. To determine the amount of tooth movement, plaster models of the maxillae were made using a silicone impression material before (day 0) and after tooth movement (days 1, 2, 3, 4, and 7). The models were scanned using a contact-type three-dimensional (3-D) measurement apparatus. Immunohistochemical staining for MMP-9, cathepsin K, and integrin subunits of alpha(v)beta3 was performed. Intergroup comparisons of the average values were conducted with a Mann-Whitney U-test for tooth movement and the number of tartrate-resistant acid phosphatase (TRAP), MMP-9, cathepsin K, and integrin subunits of alpha(v)beta3-positive cells. In the laser-irradiated group, the amount of tooth movement was significantly greater than that in the non-irradiated group at the end of the experiment (P < 0.05). Cells positively stained with TRAP, MMP-9, cathepsin K, and integrin subunits of alpha(v)beta3 were found to be significantly increased in the irradiated group on days 2-7 compared with those in the non-irradiated group (P < 0.05). These findings suggest that low-energy laser irradiation facilitates the velocity of tooth movement and MMP-9, cathepsin K, and integrin subunits of alpha(v)beta3 expression in rats.
先前有报道称，低能量激光辐射通过核因子 kappa B（RANK）/RANK 配体和巨噬细胞集落刺激因子/其受体（c-Fms）系统刺激牙齿移动速度。基质金属蛋白酶（MMP）-9、组织蛋白酶 K 和 alpha(v)beta(3)[alpha(v)beta3]整联蛋白对于破骨细胞的形成是必不可少的；因此，本研究旨在探讨低能量激光辐射对实验性牙齿移动过程中 MMP-9、组织蛋白酶 K 和 alpha(v)beta3 整联蛋白表达的影响。实验使用 50 只 6 周龄雄性 Wistar 大鼠。在大鼠磨牙上施加 10g 的总力以诱导牙齿移动。使用 Ga-Al-As 二极管激光照射移动牙周围的区域，7 天后测量牙齿移动量。为了确定牙齿移动量，在牙齿移动前（第 0 天）和移动后（第 1、2、3、4 和 7 天）使用硅橡胶印模材料制作上颌石膏模型。使用接触式三维（3-D）测量仪器对模型进行扫描。进行 MMP-9、组织蛋白酶 K 和 alpha(v)beta3 整联蛋白亚基的免疫组织化学染色。使用 Mann-Whitney U 检验对牙齿移动和抗酒石酸酸性磷酸酶（TRAP）阳性细胞、MMP-9、组织蛋白酶 K 和 alpha(v)beta3 整联蛋白亚基阳性细胞的数量进行组间平均值得比较。在激光照射组中，实验结束时牙齿移动量明显大于未照射组（P < 0.05）。与未照射组相比，照射组在第 2-7 天 TRAP、MMP-9、组织蛋白酶 K 和 alpha(v)beta3 整联蛋白亚基阳性细胞明显增加（P < 0.05）。这些发现表明，低能量激光辐射促进了大鼠牙齿移动速度和 MMP-9、组织蛋白酶 K 和 alpha(v)beta3 整联蛋白亚基的表达。
