Department of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, USA.
Opt Lett. 2010 Feb 15;35(4):532-4. doi: 10.1364/OL.35.000532.
We have developed a high-resolution molecular imaging technique, pump-probe optical coherence microscopy (PPOCM), based on the fusion of pump-probe spectroscopy and optical coherence microscopy. We have demonstrated the prototype system on a fixed human skin sample containing a nodular melanoma. The results indicate that the PPOCM can clearly provide a strong contrast between the melanotic and amelanotic regions. Potential applications of the PPOCM imaging of melanin include the early diagnosis of melanoma and the mapping of tumor margins during excision. The technique may in general be applied to any biological chromophore with a known absorption spectrum.
我们开发了一种基于泵浦探测光谱学和光学相干显微镜融合的高分辨率分子成像技术，即泵浦探测光学相干显微镜（PPOCM）。我们在一个包含结节性黑色素瘤的固定人体皮肤样本上演示了该原型系统。结果表明，PPOCM 可以清楚地提供黑色素和无黑色素区域之间的强对比。黑色素 PPOCM 成像的潜在应用包括黑色素瘤的早期诊断和切除过程中肿瘤边界的定位。该技术通常可以应用于任何具有已知吸收光谱的生物发色团。
