用于解吸电喷雾电离质谱的微流控雾化器芯片的设计、制造与测试
Design, fabrication and test of a microfluidic nebulizer chip for desorption electrospray ionization mass spectrometry.
作者信息
Sen A K, Darabi J, Knapp D R
机构信息
MEMS & Microsystems Laboratory, Department of Mechanical Engineering, University of South Carolina, 300 Main Street, Columbia, SC 29208, USA.
出版信息
Sens Actuators B Chem. 2009 Apr 2;137(2):789-796. doi: 10.1016/j.snb.2009.02.002.
This paper presents design, microfabrication, and test of a microfluidic nebulizer chip for desorption electrospray ionization mass spectrometry (DESI-MS) in proteomic analysis. The microfluidic chip is fabricated using cyclic olefin copolymer (COC) substrates. The fluidic channels are thermally embossed onto a base substrate using a nickel master and then a top substrate is thermally bonded to seal the channels. Carbon ink embossed into the top COC substrate is used to established electrical connection between the external power supply and the liquid in the channel. The microfluidic chip to external capillary connection is fabricated using Nanoport() interconnection system. Preliminary leakage test was performed to demonstrate the interconnection system is leak-free and pressure test was performed to evaluate the burst pressure. Finally, the nebulizer chip was used to perform DESI-MS for analyzing peptides (BSA and bradykinin) and reserpine on the nanoporous alumina surface. DESI-MS performance of the microfluidic nebulizer chip is compared with that obtained using a conventional DESI nebulizer.
本文介绍了一种用于蛋白质组分析中解吸电喷雾电离质谱（DESI-MS）的微流控雾化器芯片的设计、微加工和测试。该微流控芯片采用环烯烃共聚物（COC）基板制造。使用镍模板将流体通道热压印到基底基板上，然后将顶部基板热键合以密封通道。压印在顶部COC基板中的碳墨用于在外部电源和通道中的液体之间建立电连接。微流控芯片与外部毛细管的连接采用Nanoport（）互连系统制造。进行了初步泄漏测试以证明互连系统无泄漏，并进行了压力测试以评估破裂压力。最后，使用雾化器芯片在纳米多孔氧化铝表面对肽（牛血清白蛋白和缓激肽）和利血平进行DESI-MS分析。将微流控雾化器芯片的DESI-MS性能与使用传统DESI雾化器获得的性能进行了比较。
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