用于将气相色谱或毛细管液相色谱与大气压光电离质谱联用的微芯片。
Microchip for combining gas chromatography or capillary liquid chromatography with atmospheric pressure photoionization-mass spectrometry.
作者信息
Haapala Markus, Luosujärvi Laura, Saarela Ville, Kotiaho Tapio, Ketola Raimo A, Franssila Sami, Kostiainen Risto
机构信息
Division of Pharmaceutical Chemistry, Faculty of Pharmacy, P.O. Box 56, FI-00014 University of Helsinki, Finland.
出版信息
Anal Chem. 2007 Jul 1;79(13):4994-9. doi: 10.1021/ac070157a. Epub 2007 May 26.
We present a microfabricated nebulizer chip for combining atmospheric pressure photoionization-mass spectrometry (APPI-MS) with gas chromatography (GC) or capillary liquid chromatography (capLC). The chip consists of a silicon plate and a glass plate or two glass plates. The chip includes a sample inlet channel, auxiliary gas and dopant inlet, vaporizer channel, nozzle, and platinum heater. The sample eluted from the capLC or GC is mixed with auxiliary gas and dopant (toluene) in the heated vaporizer. The chip forms a confined jet of the sample vapor, which is photoionized as it exits the chip. The analytical performance of GC- and capLC-microchip APPI-MS was evaluated with some polycyclic aromatic hydrocarbons, amphetamines, and steroids. The GC-muAPPI-MS method provides high sensitivity down to 0.8 fmol, repeatability (RSD = 7.5-14%), and linearity (r = 0.9952-0.9987). The capLC-muAPPI-MS method shows high sensitivity down to 1 fmol, good repeatability (RSD = 3.6-8.1%), and linearity (r = 0.9989-0.9992).
我们展示了一种用于将大气压光电离质谱（APPI-MS）与气相色谱（GC）或毛细管液相色谱（capLC）相结合的微制造雾化器芯片。该芯片由一块硅板和一块玻璃板或两块玻璃板组成。芯片包括样品入口通道、辅助气体和掺杂剂入口、蒸发器通道、喷嘴和铂加热器。从capLC或GC洗脱的样品在加热的蒸发器中与辅助气体和掺杂剂（甲苯）混合。芯片形成样品蒸汽的受限射流，当其离开芯片时被光电离。用一些多环芳烃、苯丙胺和类固醇评估了GC-和capLC-微芯片APPI-MS的分析性能。GC-μAPPI-MS方法具有低至0.8 fmol的高灵敏度、重复性（RSD = 7.5 - 14%）和线性（r = 0.9952 - 0.9987）。capLC-μAPPI-MS方法显示出低至1 fmol的高灵敏度、良好的重复性（RSD = 3.6 - 8.1%）和线性（r = 0.9989 - 0.9992）。
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