Department of Chemistry and The Photonics Center, Boston University, Boston, Massachusetts 02215, USA.
ACS Appl Mater Interfaces. 2010 Jan;2(1):35-40. doi: 10.1021/am900822f.
Here we describe a simple yet efficient gel-matrix-assisted preparation method that improves synthetic control over the interface between inorganic nanomaterials and biopolymers and yields stable biofunctionalized silver nanoparticles. Covalent functionalization of the noble metal surface is aided by the confinement of polyethylene glycol acetic acid functionalized silver nanoparticles in thin slabs of a 1% agarose gel. The gel-confined nanoparticles can be transferred between reaction and washing media simply by immersing the gel slab in the solution of interest. The agarose matrix retains nanoparticles but is swiftly penetrated by the antibodies of interest. The antibodies are covalently anchored to the nanoparticles using conventional cross-linking strategies, and the resulting antibody functionalized nanoparticles are recovered from the gel through electroelution. We demonstrate the efficacy of this nanoparticle functionalization approach by labeling specific receptors on cellular surfaces with functionalized silver nanoparticles that are stable under physiological conditions.
在这里，我们描述了一种简单而有效的凝胶基质辅助制备方法，该方法可改善无机纳米材料与生物聚合物之间界面的可控合成，并得到稳定的生物功能化银纳米粒子。通过将聚乙二醇乙酸功能化的银纳米粒子限制在 1%琼脂糖凝胶薄片中，有助于对贵金属表面进行共价功能化。通过将凝胶薄片浸入感兴趣的溶液中，凝胶限制的纳米粒子可以在反应和洗涤介质之间简单地转移。琼脂糖基质保留纳米粒子，但被感兴趣的抗体迅速穿透。使用传统的交联策略将抗体共价固定在纳米粒子上，并且通过电泳从凝胶中回收得到抗体功能化的纳米粒子。我们通过用在生理条件下稳定的功能化银纳米粒子标记细胞表面上的特定受体，证明了这种纳米粒子功能化方法的有效性。
