Research and Post Graduate Department of Chemistry, St. Thomas College, Palai, Mahatma Gandhi University, Arunapuram P.O., Kottayam, Kerala, 686574, India.
Mater Sci Eng C Mater Biol Appl. 2013 Oct;33(7):4409-15. doi: 10.1016/j.msec.2013.06.037. Epub 2013 Jul 1.
Chitosan was functionalized with 4-((E)-2-(3-hydroxynaphthalen-2-yl)diazen-1-yl)benzoic acid by the coupling of the hydroxyl functional groups of chitosan with carboxylic acid group of the dye by DCC coupling method. The silver nanoparticles were prepared by sol-gel method of nanoparticle synthesis. Silver nanoparticle-encapsulated functionalized chitosan was prepared by the phase transfer method. The products were characterized by FTIR, UV-Vis, fluorescence and NMR spectroscopic methods and by SEM and TEM analysis. The photochemical properties of silver nanoparticle-encapsulated chitosan functionalized with 4-((E)-2-(3-hydroxynaphthalen-2-yl)diazen-1-yl)benzoic acid was studied in detail. The light-fastening properties of the chromophoric system was enhanced when attached to chitosan, and it can be further improved by the encapsulation of silver nanoparticles. The antibacterial analysis of silver nanoparticle-encapsulated functionalized chitosan was carried out against Staphylococcus aureus and Escherichia coli and against fungal species such as Aspergillus flavus and Aspergillus terreus. This study showed that silver nanoparticles-encapsulated functionalized chitosan can be used for antibacterial and antifungal applications.
壳聚糖通过 DCC 偶联法将壳聚糖的羟基官能团与染料的羧酸基团偶联，用 4-((E)-2-(3-羟基萘-2-基)重氮-1-基)苯甲酸对其进行功能化。采用纳米粒子合成的溶胶-凝胶法制备了银纳米粒子。通过相反转法制备了银纳米粒子包封的功能化壳聚糖。通过傅里叶变换红外光谱、紫外可见光谱、荧光和核磁共振光谱以及扫描电子显微镜和透射电子显微镜分析对产物进行了表征。详细研究了银纳米粒子包封的 4-((E)-2-(3-羟基萘-2-基)重氮-1-基)苯甲酸功能化壳聚糖的光化学性质。将生色团附着在壳聚糖上可以增强光稳定性，并且通过银纳米粒子的封装可以进一步提高其光稳定性。对银纳米粒子包封的功能化壳聚糖进行了针对金黄色葡萄球菌和大肠杆菌以及黄曲霉和土曲霉等真菌的抗菌分析。本研究表明，银纳米粒子包封的功能化壳聚糖可用于抗菌和抗真菌应用。
