Yang Desuo, Li Zongxiao, Allan Diwu Yubo, Fu Hanzhuo, Liao Jinfang, Wei Chunmei, Diwu Zhenjun
Department of Chemistry & Chemical Engineering, Baoji University of Arts & Science, Shaanxi, China.
Curr Chem Genomics. 2008 Nov 14;2:48-50. doi: 10.2174/1875397300802010048.
This article described the synthesis and application of 6-chloro-8-fluoro-4-methylumbelliferone phosphate (CF-MUP) in analyzing acid phosphatase activity. Compared to the existing MUP, the new coumarin phosphate, CF-MUP, demonstrateed much higher sensitivity and was more robust for detecting the activity of acid phosphatase than the classic substrate 4-methylumbelliferone phosphate (MUP). The product of enzyme reaction, 6-chloro-8-fluoro-4-methylumbelliferone (CF-MU) possesses strong fluorescence at approximately 450 nm with low pKa (4.7), high fluorescence quantum yield and pH independence in the physiological pH range. This new fluorescence dye, CF-MU, is a convenient tool for assays with buffer pH between 4.5 and 8.
本文描述了6-氯-8-氟-4-甲基伞形酮磷酸酯（CF-MUP）在酸性磷酸酶活性分析中的合成及应用。与现有的MUP相比，新型香豆素磷酸酯CF-MUP在检测酸性磷酸酶活性方面表现出更高的灵敏度，且比经典底物4-甲基伞形酮磷酸酯（MUP）更稳定。酶反应产物6-氯-8-氟-4-甲基伞形酮（CF-MU）在约450 nm处具有强荧光，pKa较低（4.7），荧光量子产率高，且在生理pH范围内与pH无关。这种新型荧光染料CF-MU是用于缓冲液pH在4.5至8之间的测定的便捷工具。
