Omene J A, Glew R H, Baig H A, Robinson D B, Brock W, Chambers J P
Afr J Med Med Sci. 1981 Mar-Jun;10(1-2):9-18.
The present report compares the performance of several colorimetric substrates presently employed in clinical laboratories for the determination of serum acid and alkaline phosphatase with that of the fluorogenic phosphatase reagent 4-methylumbelliferyl phosphate. Acid and alkaline phosphatase assays were performed on reference hospital populations in Nigeria and the United States. The results of both acid and alkaline phosphatase determinations indicate that the coefficient of variation is smaller for the fluorometric assay than for the colorimetric methods that employ either phenyl phosphate or p-nitrophenyl phosphate as the substrate. Furthermore, serum acid phosphatase determinations using 4-methylumbelliferyl phosphate identified increased serum enzyme levels in 6/6 cases of prostatic carcinoma and 9/9 cases of Gaucher's disease. The occurrence of elevated serum alkaline phosphatase levels in 24/24 cases involving liver and bone disease was also confirmed by the fluorometric alkaline phosphatase assay. In addition, sera from eight patients with the connective tissue disease, osteogenesis imperfecta, were found to contain normal levels of acid and alkaline phosphatase by the fluorometric assays. From these results it appears that the sensitive and rapid fluorometric assay procedures can be readily employed in the clinical pathology laboratory for the determination of serum acid and alkaline phosphatase levels.
本报告比较了目前临床实验室用于测定血清酸性和碱性磷酸酶的几种比色底物与荧光磷酸酶试剂4-甲基伞形酮磷酸酯的性能。在尼日利亚和美国的参考医院人群中进行了酸性和碱性磷酸酶测定。酸性和碱性磷酸酶测定的结果表明，荧光测定法的变异系数比使用磷酸苯酯或对硝基苯磷酸酯作为底物的比色法更小。此外，使用4-甲基伞形酮磷酸酯进行血清酸性磷酸酶测定时，在6例前列腺癌患者中的6例以及9例戈谢病患者中的9例中均发现血清酶水平升高。荧光碱性磷酸酶测定也证实了在24例涉及肝脏和骨骼疾病的患者中均出现血清碱性磷酸酶水平升高。此外，荧光测定法显示，8例患有结缔组织疾病成骨不全症患者的血清中酸性和碱性磷酸酶水平正常。从这些结果看来，灵敏且快速的荧光测定程序可轻易用于临床病理实验室中血清酸性和碱性磷酸酶水平的测定。
